Joshua B Wolfe

US Patent:

20160281081, Sep 29, 2016

Filed:

Mar 26, 2015

Appl. No.:

14/669125

Inventors:

- Baltimore MD, US
Joshua T. Wolfe - Bethesda MD, US

International Classification:

C12N 15/10

Abstract:

A method of directed evolution screening includes selecting a protein expression platform for an evolution target, expressing a key rupture gene configured to trigger droplet rupture, developing gene regulatory circuits to control expression of the key rupture gene as a function of performance of the evolution target, encapsulating expression components in droplets, and triggering droplet rupture by expressing a rupture agent from the key rupture gene.

US Patent:

20160215282, Jul 28, 2016

Filed:

Jan 20, 2016

Appl. No.:

15/001349

Inventors:

- Baltimore MD, US
Andrew B. Feldman - Columbia MD, US
Jared D. Evans - Ellicott City MD, US
Joshua T. Wolfe - Bethesda MD, US
David Weitz - Bolton MA, US
John Heyman - Somerville MA, US
Andrew S. Pekosz - Towson MD, US

International Classification:

C12N 15/10
C07K 16/30
C07K 16/28
C07K 16/44
C07K 16/08
C07K 16/12

Abstract:

A method for synthesizing an antiserum for rapid-turnaround therapies includes collecting antibody-secreting cells from a test subject, wherein the test subject has been exposed to a target biological agent and has produced an antibody response; selecting a subset of the antibody-secreting cells, the subset of the antibody-secreting cells producing antibodies that neutralize the target biological agent; generating variable-region-coding DNA sequences from the antibodies that neutralize the target biological agent; tagging amplicons of the variable-region-coding DNA sequences with unique nucleic acid identifiers to associate the variable-region-coding DNA sequences derived from individual ones of the subset of the antibody-secreting cells; analyzing antibody-type distribution in a natural immune response; synthesizing antibodies from the variable-region-coding DNA sequences to form synthetic antibodies; and mixing the synthetic antibodies in a proportion equal to the antibody-type distribution in the natural immune response to form the antiserum.

US Patent:

20160076094, Mar 17, 2016

Filed:

Sep 1, 2015

Appl. No.:

14/841981

Inventors:

- BALTIMORE MD, US
Jared D. Evans - Ellicott City MD, US
Thomas S. Mehoke - Washington DC, US
Joshua T. Wolfe - Bethesda MD, US

International Classification:

C12Q 1/68
C12Q 1/70

Abstract:

A method for limited input whole genome sequencing of RNA viruses includes isolating a viral RNA sample, converting the viral RNA sample to a double-stranded viral cDNA sample, constructing a double-stranded viral cDNA amplicon library from the double-stranded viral cDNA sample, and sequencing the double-stranded viral cDNA amplicon library to obtain a double-stranded viral cDNA sample sequencing read.