Judith M Fitzpatrick

US Patent:

6455047, Sep 24, 2002

Filed:

Sep 18, 1998

Appl. No.:

09/156864

Inventors:

Judith Fitzpatrick - Tenafly NJ
Regina Lenda - Wesley Hills NY

Assignee:

Serex, Inc. - Maywood NJ

International Classification:

A61K 39385

US Classification:

4241931, 4241751, 4241841, 4241941, 530405, 530409, 546131

Abstract:

A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to the hapten cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.

US Patent:

6465655, Oct 15, 2002

Filed:

Nov 22, 1999

Appl. No.:

09/444162

Inventors:

Judith Fitzpatrick - Tenafly NJ
Regina Lenda - Wesley Hills NY
Zijian Zeng - Warren NJ

Assignee:

Serex, Inc. - Maywood NJ

International Classification:

C07D21172

US Classification:

546345

Abstract:

A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md. , under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.

US Patent:

6808889, Oct 26, 2004

Filed:

Mar 16, 2000

Appl. No.:

09/526582

Inventors:

Judith Fitzpatrick - Tenafly NJ
Regina B. Lenda - Wesley Hills NY
Christopher L. Jones - Riverdale NJ

Assignee:

Serex, Inc. - Maywood NJ

International Classification:

G01N 3353

US Classification:

435 71, 435 792, 435 793, 435 794, 435810, 436518, 436174, 436808

Abstract:

A method has been developed to detect the levels of apolipoproteins A-1 and B in saliva, which is correlated with the levels of HDL and LDL in serum, respectively. In unstimulated saliva, the ration of Apo A to Apo B is correlated with the ration of HDL to LDL in serum. Albumin can be used to normalize the sample for dilution. The high degree of correlation in combination with a simple, quick test that can be performed at the site of collection provides a cost effective, patient friendly means to monitor an individuals risk of heart disease. In the preferred embodiment, saliva production is stimulated by means such as breath mint or tart solution (such as lemon) and the effect of dilution controlled by reference to albumin.

US Patent:

7147858, Dec 12, 2006

Filed:

Aug 21, 2002

Appl. No.:

10/224800

Inventors:

Judith Fitzpatrick - Englewood NJ, US
Regina Lenda - Wesley Hills NY, US

Assignee:

Serex, Inc. - Maywood NJ

International Classification:

A61K 39/00
G01N 33/66
G01N 33/68
G01N 33/72

US Classification:

4241851, 4241841, 435 71

Abstract:

A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to the hapten cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.

US Patent:

7259232, Aug 21, 2007

Filed:

Oct 27, 2000

Appl. No.:

09/697590

Inventors:

Judith Fitzpatrick - Englewood NJ, US
Paul Averback - St. Laurent, CA
Maggie Focht - Glen Rock NJ, US
Riza Bibiano - Old Bridge NJ, US

Assignee:

Nymox Pharmaceutical Corporation - Quebec

International Classification:

C07K 14/435
C07K 5/04

US Classification:

530300, 530330

Abstract:

The invention is directed to preferred repeat sequences of Neural Thread Protein (NTP), peptides, mimetics, antibodies, and nucleic acids of the preferred sequences, and diagnostic and therapeutic methods of using such preferred NTP sequences.

US Patent:

7303750, Dec 4, 2007

Filed:

Aug 21, 2002

Appl. No.:

10/224758

Inventors:

Judith Fitzpatrick - Englewood NJ, US
Regina Lenda - Wesley Hills NY, US

Assignee:

Serex, Inc. - Maywood NJ

International Classification:

A61K 39/385

US Classification:

4241841

Abstract:

A method of improving specific immune response to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In another embodiment, the peptide mimic and spacer are linked to a carrier molecule.

US Patent:

7335516, Feb 26, 2008

Filed:

Aug 21, 2002

Appl. No.:

10/224772

Inventors:

Judith Fitzpatrick - Englewood NJ, US
Regina Lenda - Wesley Hills NY, US

Assignee:

Serex, Inc. - Maywood NJ

International Classification:

G01N 33/531
A61K 38/03
C07K 17/02
C07K 16/44

US Classification:

436547, 435 71, 530345, 5303871

Abstract:

A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. Antibodies to a glycated protein have been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.

US Patent:

20030077287, Apr 24, 2003

Filed:

Aug 21, 2002

Appl. No.:

10/225227

Inventors:

Judith Fitzpatrick - Englewood NJ, US
Regina Lenda - Wesley Hills NY, US

Assignee:

Serex, Inc.

International Classification:

C12P021/04
A61K039/00

US Classification:

424/184100, 435/070210

Abstract:

A method of improving specific immune responses to smal immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to the hapten cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule. This method has been used to produce an antibody to the glycated protein HbAc, wherein the peptide mimic includes a valine modified by addition of a glucose molecule, an analog of Histidine which does not bear a charge in the immunizing structure, allows orientation of the peptide so that the immune response can be directed to the side of the peptide chain oriented oppositely to the ring, and is of a size that the conformation of the peptide mimics the conformation of the peptide in the native molecule, a leucine or an analog thereof which allows binding to an antibody preferentially recognizing Hb Ac such as 82D259, and a threonine or an analog thereof which allows binding to antibody number 82D259. In the example described below the histidine analog is 2-amino-3-flurylpropionyl, and the peptide is Fructosyl-Val-2-amino-3-furanylproprionic acid-Leu-Pro-Pro-Glu-Glu-Tyr-Tyr-Cys.