Judy Y. Chang - Hillsborough CA Nancy C. McFarland - Hillsborough CA James R. Swartz - Menlo Park CA
Assignee:
Genentech, Inc. - S. San Francisco CA
International Classification:
C07K 704 C12N 1500
US Classification:
530399
Abstract:
A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, it concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-I at concentrations sufficiently low to allow solubilization and folding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.
Method For Refolding Insoluble, Misfolded Insulin-Like Growth Factor-1 Into An Active Conformation
Judy Y. Chang - Hillsborough CA Nancy C. McFarland - Hillsborough CA James R. Swartz - Menlo Park CA
Assignee:
Genentech, Inc. - South San Francisco CA
International Classification:
C12N 1500 C07K 704
US Classification:
530408
Abstract:
A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, is concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-I at concentrations sufficiently low to allow solubilization and refolding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.
Apr 2014 to 2000 Nurse II- Staff Nurse- Medical Surgical/Telemetry/Step Down UnitVeterans Affairs Health Care System Palo Alto, CA Aug 2012 to Apr 2014 Nurse II- Staff Nurse- Spinal Cord Injury Disease Rehabilitation UnitVeterans Affairs Health Care System Palo Alto, CA Jan 2011 to Aug 2012 Nurse II- Registered Nurse Care Coordinator - Staff Nurse Ambulatory Care ClinicsWashington Hospital Washington, DC Oct 2009 to Dec 2010 Nurse I- Staff Nurse-Medical Surgical- General Medicine & Behavioral Health UnitVeterans Affairs Health Care System Palo Alto, CA Apr 2009 to Oct 2009 Nurse I- Staff Nurse- Ambulatory Care Clinics
Education:
San Francisco State University Aug 2003 to Jan 2009 Bachelor of Science in Nursing
Nov 2010 to 2000 Lead HR / Immigration SpecialistPalm, Inc Sunnyvale, CA Oct 2010 to Dec 2010 APAC Contract ConsultantJuniper Networks Sunnyvale, CA Jun 2010 to Nov 2010 Senior Executive Search Recruiting SpecialistPalm, Inc Sunnyvale, CA Mar 2009 to Jun 2010 Lead Immigration / Relocation / International Contract SpecialistPalm, Inc Sunnyvale, CA Sep 2008 to Mar 2009 Recruiting Coordinator / Executive Candidate Manager
Education:
University of Warwick Business School Warwick, RI 2008 to 2008 Spring Exchange in BusinessUniversity of California, Berkeley Berkeley, CA BA in Economics
Skills:
US Immigration, Microsoft Office, Chinese (Mandarin - Fluent)
Jul 2008 to Present Account ManagerDevon International
Jan 2008 to Jul 2008 Trade Associate/Internet Sales CoordinatorDPT Business School Philadelphia, PA Jul 2007 to Dec 2007 Admissions Representative of International StudentSaint Joseph's University ELS Program Philadelphia, PA Aug 2004 to Jun 2007 International Student Advisor
Education:
Saint Joseph's University, Haub School of Business Philadelphia, PA May 2007 Master of Science in International MarketingChien University May 2004 Bachelor of Arts in Fashion Merchandising
Group Health Cooperative East Clinic 5249 E Ter Dr, Madison, WI 53718 6082229777 (phone), 6084413961 (fax)
Education:
Medical School Northwestern University Feinberg School of Medicine Graduated: 2011
Languages:
English Spanish
Description:
Dr. Chang graduated from the Northwestern University Feinberg School of Medicine in 2011. She works in Madison, WI and specializes in Internal Medicine. Dr. Chang is affiliated with Meriter-UnityPoint Health Hospital, St Marys Hospital and University Of Wisconsin Hospital.
New York, NY and Portland, ORHolistic Psychologist/Consultant at Judy Chang Gum... Holistic Psychologist with a Private Practice in Manhattan and Portland, OR
"Some of the studies that do exist suggest that there are risks to the pregnancy from pot use," said Dr. Judy Chang, associate professor of obstetrics, gynecology and reproductive sciences at the University of Pittsburgh.
Judy Chang, head of PayPal's nonprofit group, announced that transactional fees incurred by money transfers to US 501(c)(3) organizations (or charities registered with the Canada Revenue Agency) between March 11 and April 10 will aid relief efforts in Japan.