Judy S Chang

US Patent:

52889319, Feb 22, 1994

Filed:

Dec 6, 1991

Appl. No.:

7/808451

Inventors:

Judy Y. Chang - Hillsborough CA
Nancy C. McFarland - Hillsborough CA
James R. Swartz - Menlo Park CA

Assignee:

Genentech, Inc. - S. San Francisco CA

International Classification:

C07K 704
C12N 1500

US Classification:

530399

Abstract:

A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, it concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-I at concentrations sufficiently low to allow solubilization and folding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.

US Patent:

54100267, Apr 25, 1995

Filed:

Dec 2, 1993

Appl. No.:

8/161044

Inventors:

Judy Y. Chang - Hillsborough CA
Nancy C. McFarland - Hillsborough CA
James R. Swartz - Menlo Park CA

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12N 1500
C07K 704

US Classification:

530408

Abstract:

A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, is concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-I at concentrations sufficiently low to allow solubilization and refolding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.