Julia Tait Lathrop

US Patent:

7223734, May 29, 2007

Filed:

Apr 14, 2003

Appl. No.:

10/414524

Inventors:

David J. Hammond - Laytonsville MD, US
Julia Tait Lathrop - Falls Church VA, US
Iwona Fijalkowska - Rockville MD, US

Assignee:

The American National Red Cross - Falls Church VA

International Classification:

A61K 38/08
C07K 7/04
C07K 2/00

US Classification:

514 17, 514 2, 530300, 530329

Abstract:

The invention provides an isolated or purified peptide that binds at least one plasma protein. In one embodiment, the isolated or purified peptide binds to fibrinogen, comprises no more than 10 amino acids, and comprises an amino acid sequence Xaa-Xaa-Xaa-Xaa-Xaa, an amino acid sequence Gly-Xaa-Arg-Xaa, or an amino acid sequence selected from specific amino acid sequences provided herein. Alternatively, the isolated or purified protein binds to αl proteinase inhibitor and/or a protein complex comprising Apo-A1 lipoprotein and paraoxonase. The peptide comprises no more than 10 amino acids and comprises an amino acid sequence Xaa-Xaa-Xaa-His-Xaa-Xaa, and amino acid sequence His-Xaa-Xaa-Xaa-Xaa-Xaa, or an amino acid sequence selected from specific amino acid sequences provided herein. In addition, the invention provides isolated or purified peptide that binds to von Willebrand Factor. The peptide comprises an amino acid sequence Xaa-Xaa-Xaa, an amino acid sequence Tyr-Leu-Xaa-Xaa-Xaa-Thr, or an amino acid sequence selected from specific amino acid sequences provided herein.

US Patent:

7863411, Jan 4, 2011

Filed:

Dec 3, 2003

Appl. No.:

10/727335

Inventors:

David J. Hammond - Laytonsville MD, US
Julia T. Lathrop - Falls Church VA, US
Larisa Cervenakova - Rockville MD, US
Ruben G. Carbonell - Raleigh NC, US

Assignee:

Pathogen Removal and Diagnostics Technologies Inc. - Wilmington DE
North Carolina State University - Raleigh NC

International Classification:

C07K 5/00

US Classification:

530300

Abstract:

Ligands that bind to prion proteins and methods for using the ligands for detecting or removing a prion protein from a sample, such as a biological fluid or an environmental sample. The ligands are capable of binding to one or more forms of prion protein including cellular prion protein (PrPc), infectious prion protein (PrPsc), and recombinant prion protein (PrPr). Prions from various species, including humans and hamsters, are bound by the ligands. Also provided is a method of treating or retarding the development of a prion-associated pathology in a subject.

US Patent:

8604161, Dec 10, 2013

Filed:

Feb 22, 2008

Appl. No.:

12/035917

Inventors:

David J. Hammond - Laytonsville MD, US
Julia T. Lathrop - Falls Church VA, US
Larisa Cervenakova - Rockville MD, US
Ruben G. Carbonell - Raleigh NC, US

Assignee:

Pathogen Removal and Diagnostic Technologies Inc. - Wilmington DE
North Carolina State University - Raleigh NC

International Classification:

C07K 5/00

US Classification:

530300

Abstract:

Ligands that bind to prion proteins and methods for using the ligands for detecting or removing a prion protein from a sample, such as a biological fluid or an environmental sample. The ligands are capable of binding to one or more forms of prion protein including cellular prion protein (PrPc), infectious prion protein (PrPsc), and recombinant prion protein (PrPr). Prions from various species, including humans and hamsters, are bound by the ligands. Also provided is a method of treating or retarding the development of a prion-associated pathology in a subject.

US Patent:

20030211471, Nov 13, 2003

Filed:

Apr 14, 2003

Appl. No.:

10/414523

Inventors:

David Hammond - Laytonsville MD, US
Julia Lathrop - Falls Church VA, US

Assignee:

The American National Red Cross - Falls Church VA

International Classification:

C12Q001/70
C12Q001/68
G01N033/53
G01N033/567
G01N033/569
G01N033/554
G01N033/537
G01N033/543

US Classification:

435/005000, 435/007200, 435/006000, 435/007310, 435/007320, 435/007930

Abstract:

The invention provides a method of characterizing a target that binds to a ligand. The method comprises providing ligands, optionally attached to a support, and contacting the ligands with targets to allow at least one target to bind to at least one ligand. The method further comprises immobilizing the resulting complexes in a first matrix, such that each complex has a different position within the first matrix, and transferring the target of the complex to a second matrix. The position of the target within the second matrix corresponds to the position of the ligand-support complex within the first matrix. The target on the second matrix is then detected.

US Patent:

20040101830, May 27, 2004

Filed:

Jun 20, 2003

Appl. No.:

10/601032

Inventors:

David Hammond - Laytonsville MD, US
Julia Lathrop - Falls Church VA, US
Jolly Sarkar - Olney MD, US
Liliana Gheorghiu - Bethesda MD, US

Assignee:

The American National Red Cross - Falls Church VA

International Classification:

C12Q001/70
C12Q001/68
G01N033/53
G01N033/567
G01N033/569
G01N033/554

US Classification:

435/005000, 435/006000, 435/007100, 435/007310, 435/007320, 435/007200

Abstract:

A method of screening a mixture for active entities, which method comprises: providing a plurality of ligands, wherein each ligand is attached to a support to form a plurality of ligand-support complexes, contacting the ligand-support complexes with a mixture comprising a plurality of entities under conditions that allow at least one entity to bind to at least one ligand-support complex, thereby forming at least one entity-ligand-support complex, separating at least one entity-ligand-support complex from the unbound entities, assaying at least one entity of at least one entity-ligand-support complex for an activity, detecting the activity, and selecting at least one entity-ligand-support-complex having the entity, which exhibited the detected activity, whereupon a mixture is screened for active entities; and related methods.

US Patent:

20050032138, Feb 10, 2005

Filed:

Apr 14, 2004

Appl. No.:

10/823888

Inventors:

Julia Lathrop - Falls Church VA, US
David Hammond - Laytonsville MD, US
Larisa Cervenakova - Rockville MD, US
Liliana Gheorghiu - Cambridge MA, US
Oksana Yakovleva - Derwood MD, US

International Classification:

G01N033/53
G01N033/537
G01N033/543

US Classification:

435007920

Abstract:

A method of identifying a ligand specific for a structural isoform of a protein by the binding of the structural isoform to a ligand on a support, a direct positional transfer of the structural isoform and a control isoform between two or more different solid or semi-solid supports and detection of at least one of the isoforms on each support, thus allowing for subtractive identification techniques to be used to identify the subset of ligands, or a ligand, specific for the structural isoform.

US Patent:

20060275753, Dec 7, 2006

Filed:

Jun 19, 2006

Appl. No.:

11/454799

Inventors:

David Hammond - Laytonsville MD, US
Julia Lathrop - Falls Church VA, US

International Classification:

C12Q 1/70
C12Q 1/68
G01N 33/53
G01N 33/92
G01N 33/00

US Classification:

435005000, 435007100, 435006000, 436086000, 204450000, 436071000

Abstract:

The invention provides a method of binding multiple targets in samples by binding to multiple ligands. The method comprises providing ligands attached to a support, and contacting the ligands with targets to produce at least two target-ligand-support complexes. The method further comprises removal of non-bound targets followed by elution of the bound targets. The eluted targets are present in concentrations of a particular analyte that is a function of their comparative concentrations in different samples. Furthermore, the mixture is enriched for trace components.

US Patent:

20060275829, Dec 7, 2006

Filed:

Jun 19, 2006

Appl. No.:

11/454800

Inventors:

David Hammond - Laytonsville MD, US
Julia Lathrop - Falls Church VA, US

International Classification:

C40B 30/06
C40B 40/10

US Classification:

435007100

Abstract:

The present invention relates to solid-phase combinatorial peptide libraries synthesized on chromatography beads and their use to prepare samples for proteomic investigations.