Karen Yue Ding

US Patent:

20040071596, Apr 15, 2004

Filed:

Mar 5, 2003

Appl. No.:

10/221527

Inventors:

Dianna Innerst - San Jose CA, US
Adonis Kassinos - San Jose CA, US
C Wooley - Roanoke VA, US
Brett Wilmarth - Tracy CA, US
William Sell - Petaluma CA, US
Emi Zychlinsky - Palo Alto CA, US
Donald Marino - Scotts Valley CA, US
Sandy Yamada - San Jose CA, US
Renee Ryan - San Jose CA, US
Karen Ding - Fremont CA, US
Michael Zatzke - San Jose CA, US

International Classification:

G01N021/29

US Classification:

422/082050

Abstract:

An enclosed chamber is used in a system for screening of a liquid specimen through binding assays. The enclosed chamber includes an inlet, an outlet, and a plurality of discrete reactant containing wells communicated by a common reagent flow path between the inlet and the outlet. A transparent member or coverslip defines on an inside thereof the plurality of wells. Each well has a bottom for receiving an allergent/antigen/reactant, which emits light upon reacting. The coverslip can optionally define at least one lens at each well. A bottom encloses the plurality of wells and defines between the inlet and the outlet a common reagent flow path between the inlet and the outlet. This bottom defines for each of the plurality of wells a flow-diverting member. An opaque partition is disclosed for surrounding the individual lenses and generally isolating the light path from each well.