Karl B Yoder

US Patent:

8105554, Jan 31, 2012

Filed:

Mar 11, 2005

Appl. No.:

11/078196

Inventors:

Tanya S. Kanigan - Lexington MA, US
Steve Smith - Arlington MA, US
John Linton - Lincoln MA, US
Robert Hess - Arlington MA, US
Karl Yoder - Stoneham MA, US
Colin Brennan - Marblehead MA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

B01L 3/00

US Classification:

422504, 422 67, 422400, 506 33, 137 67

Abstract:

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

US Patent:

8545772, Oct 1, 2013

Filed:

Dec 23, 2011

Appl. No.:

13/336902

Inventors:

Tanya Kanigan - Winchester MA, US
Steve Smith - Arlington MA, US
John Linton - Lincoln MA, US
Robert Hess - Arlington MA, US
Karl Yoder - Stoneham MA, US
Colin Brennan - Marblehead MA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

G01N 1/00
B01L 3/00

US Classification:

422503, 422 681, 422277, 422278, 422504, 422552, 436176, 506 16, 506 39, 137 1, 137803

Abstract:

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.

US Patent:

20040208792, Oct 21, 2004

Filed:

Dec 22, 2003

Appl. No.:

10/744580

Inventors:

John Linton - Lincoln MA, US
Karl Yoder - Stoneham MA, US
Robert Hess - Arlington MA, US
Leila Hasan - Boston MA, US
Robert Ellis - Burlington MA, US
Tanya Kanigan - Cambridge MA, US
Kristine Friesen - Cambridge MA, US
Arrin Katz - Somerville MA, US
Colin Brenan - Marblehead MA, US
Tom Morrison - Winchester MA, US
Javier Garcia - Lexington MA, US

International Classification:

B01L003/00
C12M003/00

US Classification:

422/099000, 422/102000, 435/286100

Abstract:

A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

US Patent:

20060094108, May 4, 2006

Filed:

Sep 15, 2005

Appl. No.:

11/227425

Inventors:

Karl Yoder - Stoneham MA, US
Colin Brenan - Marblehead MA, US
John Linton - Lincoln MA, US
Leila Hasan - Brookline MA, US
Robert Ellis - Burlington MA, US
Arrin Katz - Cambridge MA, US
Tom Morrison - Winchester MA, US
Jorge Fonseca - Boston MA, US

International Classification:

C12M 1/34

US Classification:

435287200, 435293100, 435303100

Abstract:

A system for thermal cycling a plurality of samples. The system includes a case having a fluid-tight cavity defining an interior volume. A microfluidic array is disposed in the interior volume, the array including a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces. A thermal cycler having at least one thermally controlled surface is adapted to thermally contact the case.

US Patent:

20060105453, May 18, 2006

Filed:

Aug 4, 2005

Appl. No.:

11/198882

Inventors:

Colin Brenan - Marblehead MA, US
Jamie Cho - Stoughton MA, US
Javier Garcia - Lexington MA, US
Robert Hess - Arlington MA, US
Tanya Kanigan - Winchester MA, US
Arrin Katz - Cambridge MA, US
Namyong Kim - Singapore, SG
John Linton - Lincoln MA, US
Shailesh Srivastava - Medway MA, US
Karl Yoder - Stoneham MA, US

International Classification:

C12N 5/00

US Classification:

435325000, 435410000

Abstract:

A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.

US Patent:

20090062134, Mar 5, 2009

Filed:

Oct 3, 2008

Appl. No.:

12/245027

Inventors:

John Linton - Lincoln MA, US
Karl Yoder - Stoneham MA, US
Robert Hess - Arlington MA, US
Leila Hasan - San Francisco CA, US
Robert Ellis - Burlington MA, US
Tanya S. Kanigan - Cambridge MA, US
Kristine Friesen - Cambridge MA, US
Arrin Katz - Somerville MA, US
Colin Brenan - Marblehead MA, US
Tom Morrison - Winchester MA, US
Javier Garcia - Lexington MA, US

Assignee:

BIOTROVE, INC. - Woburn MA

International Classification:

C40B 30/00

US Classification:

506 7

Abstract:

A method of conducting an assay on a plurality of samples is provided. The method includes the steps of performing an assay at each sample site in a sample array having greater than 100 sample sites simultaneously illuminating each sample site using one or more LEDs, and simultaneously imaging each of the sample sites to produce imaging data pertinent to the optical effect of each site. Each assay provides an optical effect.

US Patent:

20090062152, Mar 5, 2009

Filed:

Oct 1, 2008

Appl. No.:

12/243503

Inventors:

John Linton - Lincoln MA, US
Karl Yoder - Stoneham MA, US
Robert Hess - Arlington MA, US
Leila Hasan - Boston MA, US
Robert Ellis - Burlington MA, US
Tanya S. Kanigan - Cambridge MA, US
Kristine Friesen - Cambridge MA, US
Arrin Katz - Cambridge MA, US
Colin Brenan - Marblehead MA, US
Tom Morrison - Winchester MA, US
Javier Garcia - Lexington MA, US

Assignee:

BIOTROVE, INC. - Woburn MA

International Classification:

C40B 60/12

US Classification:

506 39

Abstract:

A thermal cycling device and a method of thermal cycling are provided. A thermal cycling device includes a fluid device system, a case, and a cycling head. The fluid delivery system develops a flow of controlled-temperature fluid. The case has a fluid-tight cavity for holding a microfluidic array. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces. The cycling head holds the case and delivers the flow of fluid over the case.

US Patent:

20110003699, Jan 6, 2011

Filed:

Dec 18, 2009

Appl. No.:

12/642715

Inventors:

Karl Yoder - Stoneham MA, US
Colin Brenan - Marblehead MA, US
John Linton - Lincoln MA, US
Leila Hasan - San Francisco CA, US
Robert Ellis - Half Moon Bay CA, US
Arrin Katz - Cambridge MA, US
Tom Morrison - Winchester MA, US
Jorge Fonseca - Boston MA, US

Assignee:

BioTrove, Inc. - Carlsbad CA

International Classification:

C40B 30/00
C40B 60/14
C40B 60/08
C40B 50/06

US Classification:

506 7, 506 40, 506 37, 506 26

Abstract:

A system for thermal cycling a plurality of samples. The system includes a case having a fluid-tight cavity defining an interior volume. A microfluidic array is disposed in the interior volume, the array including a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces. A thermal cycler having at least one thermally controlled surface is adapted to thermally contact the case.