Katherine P Collier

US Patent:

6482628, Nov 19, 2002

Filed:

Oct 23, 1998

Appl. No.:

09/178173

Inventors:

Ayrookaran J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - Belmont CA
Andre C. Baeck - Bonheiden, BE

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 956

US Classification:

435221, 435 691, 435220, 43525231, 4353201, 435471, 510392, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of subtilisin.

US Patent:

6562340, May 13, 2003

Filed:

Aug 16, 1995

Appl. No.:

08/507362

Inventors:

Michael R. Bedford - Marlborough, GB
Andrew J. Morgan - Marlborough, GB
Timothy Fowler - Belmont CA
Kathleen A. Clarkson - San Francisco CA
Michael Ward - San Francisco CA
Katherine D. Collier - Redwood City CA
Edmund A Larenas - San Carlos CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

A61K 3847

US Classification:

424 9461, 424 941, 424 942, 426615

Abstract:

The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, tricale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

US Patent:

6620605, Sep 16, 2003

Filed:

Jul 27, 2001

Appl. No.:

09/916494

Inventors:

Timothy Fowler - San Carlos CA
Kathleen A. Clarkson - San Francisco CA
Michael Ward - San Francisco CA
Katherine D. Collier - Redwood City CA
Edmund Larenas - Moss Beach CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12N 942

US Classification:

435209, 435263, 435264, 2 1

Abstract:

Improved methods of treating cellulose containing fabrics with cellulase comprising contacting the cellulose fabrics with truncated cellulase enzyme. Treatment of cellulose containing fabrics with cellulase core domains of the invention are disclosed as offering specific advantages of reduced redeposition of dye and increased abrasion.

US Patent:

6673590, Jan 6, 2004

Filed:

Oct 23, 1998

Appl. No.:

09/177353

Inventors:

Ayrookaran .J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - Belmont CA

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 954

US Classification:

435220, 435 691, 435221, 435222, 4352523, 4353201, 435471, 570392, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease.

US Patent:

6815193, Nov 9, 2004

Filed:

Nov 2, 2001

Appl. No.:

10/033325

Inventors:

Ayrookaran J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - Belmont CA
André C. Baeck - Bonheiden, BE

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12N 956

US Classification:

435220, 435221, 435222, 435 691, 4352523, 4353201, 435471, 510392, 510400, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of subtilisin.

US Patent:

6927055, Aug 9, 2005

Filed:

Aug 27, 2002

Appl. No.:

10/228572

Inventors:

Ayrookaran J. Poulose - Belmont CA, US
Volker Schellenberger - Palo Alto CA, US
Christian Paech - Daly City CA, US
Joanne Nadherny - San Francisco CA, US
Donald P. Naki - San Francisco CA, US
Katherine D. Collier - Redwood City CA, US
Robert M. Caldwell - Belmont CA, US
André C. Baeck - Bonheiden, BE

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12N009/50
C12N009/52
C12N009/54
C12N009/56
C11D003/386

US Classification:

435219, 435220, 435221, 435222, 435 691, 4352523, 4353201, 510300, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of subtilisin.

US Patent:

7005128, Feb 28, 2006

Filed:

Mar 4, 2003

Appl. No.:

10/379978

Inventors:

Michael R. Bedford - Marlborough, GB
Andrew J. Morgan - Marlborough, GB
Timothy Fowler - Belmont CA, US
Kathleen A. Clarkson - San Francisco CA, US
Michael Ward - San Francisco CA, US
Katherine D. Collier - Redwood City CA, US
Edmund A Larenas - San Carlos CA, US

Assignee:

Genencor International, Inc. - Palo Alto CA
Finnfeeds International, Ltd. - Marlborough

International Classification:

A61K 38/47
A61K 38/43
A61K 38/54
A23K 1/00

US Classification:

424 9461, 424 941, 424 942, 426615

Abstract:

The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0–20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0–20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0–20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, triticale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

US Patent:

7122334, Oct 17, 2006

Filed:

Jul 26, 2004

Appl. No.:

10/899751

Inventors:

Volker Schellenberger - Palo Alto CA, US
Donald P. Naki - San Francisco CA, US
Katherine D. Collier - Redwood City CA, US
Joanne Nadherny - San Francisco CA, US

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12Q 1/34

US Classification:

435 18, 435 23

Abstract:

A microtiter method for assaying the wash performance of new enzymes and/or new detergent formulations is described.