Katherine Diane Collier

US Patent:

6436696, Aug 20, 2002

Filed:

Dec 27, 1999

Appl. No.:

09/472660

Inventors:

You-lo Hsieh - Davis CA
Mary Michelle Hartzell - Logan UT
Matthew G. Boston - San Carlos CA
Kathleen A. Clarkson - San Francisco CA
Katherine D. Collier - Redwood City CA
Thomas P. Graycar - Pacifica CA
Edmund A. Larenas - Moss Beach CA

Assignee:

The Regents of the University of California - Oakland CA
Genencor International, Inc. - Rochester NY

International Classification:

C12S 1100

US Classification:

435263, 8401, 811554, 8DIG 4

Abstract:

Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55Â C. for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0. 01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25Â C. ).

US Patent:

6482628, Nov 19, 2002

Filed:

Oct 23, 1998

Appl. No.:

09/178173

Inventors:

Ayrookaran J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - Belmont CA
Andre C. Baeck - Bonheiden, BE

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 956

US Classification:

435221, 435 691, 435220, 43525231, 4353201, 435471, 510392, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of subtilisin.

US Patent:

6562340, May 13, 2003

Filed:

Aug 16, 1995

Appl. No.:

08/507362

Inventors:

Michael R. Bedford - Marlborough, GB
Andrew J. Morgan - Marlborough, GB
Timothy Fowler - Belmont CA
Kathleen A. Clarkson - San Francisco CA
Michael Ward - San Francisco CA
Katherine D. Collier - Redwood City CA
Edmund A Larenas - San Carlos CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

A61K 3847

US Classification:

424 9461, 424 941, 424 942, 426615

Abstract:

The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, tricale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

US Patent:

6610642, Aug 26, 2003

Filed:

Aug 6, 2001

Appl. No.:

09/922678

Inventors:

Chanchal Kumar Ghosh - West Chester OH
André Cesar Baeck - Bonheiden, BE
Ryohei Ohtani - UedaNaka-machi, JP
Alfred Busch - Londerzeel, BE
Michael Stanford Showell - Cincinnati OH
Ayrookaran J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - San Carlos CA

Assignee:

The Procter and Gamble Company - Cincinnati OH

International Classification:

C11D 742

US Classification:

510392, 510305, 510320, 510226, 510130, 510530, 435219, 435220, 435221, 435222

Abstract:

The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of ; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a subtitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of ; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of ; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.

US Patent:

6620605, Sep 16, 2003

Filed:

Jul 27, 2001

Appl. No.:

09/916494

Inventors:

Timothy Fowler - San Carlos CA
Kathleen A. Clarkson - San Francisco CA
Michael Ward - San Francisco CA
Katherine D. Collier - Redwood City CA
Edmund Larenas - Moss Beach CA

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12N 942

US Classification:

435209, 435263, 435264, 2 1

Abstract:

Improved methods of treating cellulose containing fabrics with cellulase comprising contacting the cellulose fabrics with truncated cellulase enzyme. Treatment of cellulose containing fabrics with cellulase core domains of the invention are disclosed as offering specific advantages of reduced redeposition of dye and increased abrasion.

US Patent:

6673590, Jan 6, 2004

Filed:

Oct 23, 1998

Appl. No.:

09/177353

Inventors:

Ayrookaran .J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - Belmont CA

Assignee:

Genencor International, Inc. - Rochester NY

International Classification:

C12N 954

US Classification:

435220, 435 691, 435221, 435222, 4352523, 4353201, 435471, 570392, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease.

US Patent:

6815193, Nov 9, 2004

Filed:

Nov 2, 2001

Appl. No.:

10/033325

Inventors:

Ayrookaran J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - Belmont CA
André C. Baeck - Bonheiden, BE

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12N 956

US Classification:

435220, 435221, 435222, 435 691, 4352523, 4353201, 435471, 510392, 510400, 536 232

Abstract:

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of subtilisin.

US Patent:

6831053, Dec 14, 2004

Filed:

Apr 20, 2000

Appl. No.:

09/529904

Inventors:

Chanchal Kumar Ghosh - West Chester OH
André Cesar Baeck - Bonheiden, BE
Ryohei Ohtani - Naka-machi, JP
Alfred Busch - Londerzeel, BE
Michael Stanford Showell - Cincinnati OH
Ayrookaran J. Poulose - Belmont CA
Volker Schellenberger - Palo Alto CA
Christian Paech - Daly City CA
Joanne Nadherny - San Francisco CA
Donald P. Naki - San Francisco CA
Katherine D. Collier - Redwood City CA
Robert M. Caldwell - San Carlos CA

Assignee:

The Procter Gamble Company - Cincinnati OH

International Classification:

C11D 300

US Classification:

510392, 392305, 392300, 435221, 435219, 435222

Abstract:

The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.