Kathleen M Valentine

US Patent:

20100285608, Nov 11, 2010

Filed:

Jul 18, 2008

Appl. No.:

12/669626

Inventors:

Kathleen G. Valentine - Ivyland PA, US
Michael Marlow - Fort Lee NJ, US
Andrew J. Wand - Philadelphia PA, US
Kendra K. Frederick - Boston MA, US

Assignee:

The Trustee of the University of Pennsylvania - Philadelphia PA

International Classification:

G01N 33/50

US Classification:

436501

Abstract:

The present invention provides methods for the determination of the degree of molecular recognition of a protein with a ligand, including a first protein with a second protein. The methods may comprise determining the squared generalized order parameter (hereinafter, O) for at least one intramolecular bond of the first protein. The protein is then formed into a complex with a ligand. The value or values of O2 for the said at least one bond of the protein is then determined while the protein and the ligand are in the complex. The O value or values determined for the protein while the protein and the ligand are in a complex are compared or related to the O value or values determined for the uncomplexed protein.