Kathryn Ann Nelson

US Patent:

20130053546, Feb 28, 2013

Filed:

Mar 11, 2011

Appl. No.:

13/582044

Inventors:

Kathryn Margaret Nelson - DeForest WI, US
Michael William Mosesson - Milwaukee WI, US
Anthony Pusateri - Hillsborough NJ, US
Erik Jordahl Forsberg - Fitchburg WI, US

Assignee:

PHARMING INTELLECTUAL PROPERTY BV - Leiden

International Classification:

C12P 21/00
C07K 14/75

US Classification:

530382, 800 7

Abstract:

The invention relates to the production of recombinant human fibrinogen (rhFib) in the milk of transgenic mammalian animals. This production and subsequent purification process is generally hampered by the occurrence of so-called ‘clots’ and ‘flakes’ in the milk which, in severe cases, may prevent the cow from being milked at all, resulting in a halt of lactation. These clots and flakes occur because of the expression of the fibrinogen protein, which is a factor that is normally involved in blood clotting. The invention relates to solving this milk clotting problem by treating the (lactating) animals with anticoagulants such as coumarins. A preferred anticoagulant that is used in the methods of the present invention is warfarin.