Katie Lee Anderson

US Patent:

20160033504, Feb 4, 2016

Filed:

Mar 18, 2014

Appl. No.:

14/776945

Inventors:

- Bothell WA, US
Benjamin H. Dutzar - Seattle WA, US
Leon F. Garcia-Martinez - Woodinville WA, US
Katie Anderson - Kirkland WA, US
Ethan W. Ojala - Snohomish WA, US
John A. Latham - Seattle WA, US
Jens Billgren - Seattle WA, US
Anne Elisabeth Carvalho Jensen - Snohomish WA, US

International Classification:

G01N 33/569

Abstract:

Methods of identifying antigen-specific antibody-secreting and antibody-forming cells, such as antigen-specific B cells, and methods for cloning the antigen-specific antibody sequences of the antibody produced by these cells are provided. In particular, the methods include enriching B cells for antigen-specific B cells, culturing the antigen-specific B cells to generate clonal B cell populations, detecting clonal B cells that produce a single antigen-specific antibody, optionally screening the clonal B cell populations for functional activity, staining and sorting the cells to isolate the antigen-specific B cells, sequencing the nucleic acids encoding the antigen-specific antibody sequences, expressing the sequences to produce an antibody, isolating the antibody and screening the antibody for antigen recognition. The methods provide improved enrichment and selection of antigen-specific antibody-secreting and antibody-forming cells, which enhances recovery of antigen-specific antibodies.

US Patent:

20140287952, Sep 25, 2014

Filed:

Mar 18, 2014

Appl. No.:

14/217594

Inventors:

- LAS VEGAS NV, US
JENS BILLGREN - SEATTLE WA, US
ANNE ELISABETH CARVALHO JENSEN - SNOHOMISH WA, US
BENJAMIN H. DUTZAR - SEATTLE WA, US
LEON F. GARCIA-MARTINEZ - WOODINVILLE WA, US
KATIE ANDERSON - KIRKLAND WA, US
ETHAN WAYNE OJALA - SNOHOMISH WA, US
JOHN A. LATHAM - SEATTLE WA, US

Assignee:

ALDERBIO HOLDINGS LLC - LAS VEGAS NV

International Classification:

G01N 33/569
C07K 16/40
C07K 16/22
G01N 33/68
C07K 16/26

US Classification:

506 9, 435 724, 435 612, 435 792, 435326, 435344, 435336, 435335, 435338

Abstract:

Methods of identifying antigen-specific antibody-secreting and antibody-forming cells, such as antigen-specific B cells, and methods for cloning the antigen-specific antibody sequences of the antibody produced by these cells are provided. In particular, the methods include enriching B cells for antigen-specific B cells, culturing the antigen-specific B cells to generate clonal B cell populations, detecting clonal B cells that produce a single antigen-specific antibody, optionally screening the clonal B cell populations for functional activity, staining and sorting the cells to isolate the antigen-specific B cells, sequencing the nucleic acids encoding the antigen-specific antibody sequences, expressing the sequences to produce an antibody, isolating the antibody and screening the antibody for antigen recognition. The methods provide improved enrichment and selection of antigen-specific antibody-secreting and antibody-forming cells, which enhances recovery of antigen-specific antibodies.