Keith D Walker

US Patent:

6870075, Mar 22, 2005

Filed:

Oct 10, 2000

Appl. No.:

09/685403

Inventors:

Peter R. Beetham - Carlsbad CA, US
Patricia L. Avissar - East Brunswick NJ, US
Keith A. Walker - San Diego CA, US
Richard A. Metz - Lawrenceville NJ, US

Assignee:

Valigen (US), Inc. - Newton PA

International Classification:

C12N015/09
C12N015/82

US Classification:

800278, 435430, 435468, 435470, 800292, 800300, 8003001

Abstract:

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e. g. , glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide.

US Patent:

8106259, Jan 31, 2012

Filed:

May 11, 2009

Appl. No.:

12/463891

Inventors:

Peter R. Beetham - Carlsbad CA, US
Keith A. Walker - San Diego CA, US
Patricia L. Avissar - New Brunswick NJ, US

Assignee:

Cibus US LLC - San Diego CA

International Classification:

C12N 15/82

US Classification:

800295, 800306

Abstract:

Mixed duplex oligonucleotides (MDON) are used to effect site-specific genetic alterations in a target DNA sequence of a plant. The MDON are introduced by electroporation into microspores. Thereafter, plants having a desired genetic alteration are produced by germinating the microspores.

US Patent:

20050177899, Aug 11, 2005

Filed:

Feb 4, 2005

Appl. No.:

11/051955

Inventors:

Peter Beetham - Carlsbad CA, US
Patricia Avissar - East Brunswick NJ, US
Keith Walker - San Diego CA, US
Richard Metz - Lawrenceville NJ, US

International Classification:

A01H001/00
C12N015/82

US Classification:

800278000

Abstract:

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene.

US Patent:

20060162024, Jul 20, 2006

Filed:

Jan 24, 2006

Appl. No.:

11/338586

Inventors:

Peter Beetham - Carlsbad CA, US
Keith Walker - San Diego CA, US
Patricia Avissar - New Brunswick NJ, US

International Classification:

A01H 5/00
C12N 15/82
A01H 1/00

US Classification:

800287000, 800306000, 435468000

Abstract:

Mixed duplex oligonucleotides (MDON) are used to effect site-specific genetic alterations in a target DNA sequence of a plant. The MDON are introduced by electroporation into microspores. Thereafter, plants having a desired genetic alteration are produced by germinating the microspores.

US Patent:

20080256668, Oct 16, 2008

Filed:

Nov 16, 2007

Appl. No.:

11/941666

Inventors:

PETER R. BEETHAM - Carlsbad CA, US
Patricia L. Avissar - East Brunswick NJ, US
Keith A. Walker - San Diego CA, US
Richard A. Metz - Lawrenceville NJ, US

International Classification:

A01H 5/00

US Classification:

8003001, 800300

Abstract:

The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphoniorethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phophonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene.

US Patent:

20120060243, Mar 8, 2012

Filed:

Sep 24, 2009

Appl. No.:

13/121008

Inventors:

Peter Beetham - Carlsbad CA, US
Dale Carlson - Apex NC, US
Greg Gocal - San Diego CA, US
John McElver - Durham NC, US
James Pearce - San Diego CA, US
Christian Schopke - San Diego CA, US
Bijay Singh - Cary NC, US
Keith Walker - San Diego CA, US

International Classification:

A01H 5/00
A01H 5/10
C12N 15/54

US Classification:

800300, 536 232

Abstract:

The invention provides transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit, expression vectors, plants comprising the nucleic acids encoding the AHASL subunits containing one, two, or more mutations, plants comprising such AHASL subunit mutant nucleic acids, methods for making and using the same, and methods of controlling weeds.

US Patent:

20180051296, Feb 22, 2018

Filed:

Oct 16, 2017

Appl. No.:

15/785297

Inventors:

- SAN DIEGO CA, US
- AD KAPELLE, NL
Peter R. BEETHAM - Carlsbad CA, US
Keith A. WALKER - San Diego CA, US

Assignee:

CIBUS US LLC - SAN DIEGO CA
CIBUS EUROPE B.V. - AD KAPELLE

International Classification:

C12N 15/82
C12N 9/88

Abstract:

Provided are compositions and methods relating to gene and/or protein mutations in plants. In certain embodiments, the disclosure relates to mutations in the allene oxide synthase 2 gene (i.e., AOS2). In some embodiments the disclosure relates to plants that are pathogen resistant.

US Patent:

20160145638, May 26, 2016

Filed:

Mar 14, 2014

Appl. No.:

14/776647

Inventors:

- San Diego CA, US
- AD Kapelle, NL
Peter R. BEETHAM - Carlsbad CA, US
Keith A. WALKER - San Diego CA, US

Assignee:

CIBUS US LLC - San Diego CA
CIBUS EUROPE B.V. - AD Kapelle

International Classification:

C12N 15/82

Abstract:

Provided are compositions and methods relating to gene and/or protein mutations in plants. In certain embodiments, the disclosure relates to mutations in the allene oxide synthase 2 gene (i.e., AOS2). In some embodiments the disclosure relates to plants that are pathogen resistant.