Kenneth Alden Browne

US Patent:

6902891, Jun 7, 2005

Filed:

Dec 15, 2000

Appl. No.:

09/736151

Inventors:

Ali Laayoun - Lyons, FR
Lionel Menou - Lyons, FR
Christelle Tora - Lyons, FR
Aloke R. Banerjee - San Diego CA, US
Michael M. Becker - San Diego CA, US
Kenneth A. Browne - San Diego CA, US
Matthew C. Friedenberg - San Diego CA, US
Fred F. Hajjar - San Diego CA, US

Assignee:

Bio Merieux - Marcy l' Etoile
Gen-Probe Incorporated - San Diego CA

International Classification:

C12Q001/68
C12P019/34
C07H021/04
C07D225/00
C07D259/00

US Classification:

435 6, 435 912, 536 253, 536 2531, 540465, 540474, 556 1, 556 6

Abstract:

A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.

US Patent:

7070933, Jul 4, 2006

Filed:

Mar 14, 2003

Appl. No.:

10/388918

Inventors:

Kenneth A. Browne - Poway CA, US

Assignee:

Gen-Probe Incorporated - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00

US Classification:

435 6, 435 911, 536 231, 536 243, 536 2433, 536 253, 536 2532

Abstract:

Unitary hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. One arm of the invented parallel-stem hybridization probe has a backbone polarity opposite that of the target-complementary loop sequence of the probe. Rather than interacting in an antiparallel fashion, the arms of parallel-stem hybridization probes interact in a parallel fashion. The arms of the invented dual inversion probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

US Patent:

7582470, Sep 1, 2009

Filed:

Jul 17, 2003

Appl. No.:

10/621803

Inventors:

Kenneth A. Browne - Poway CA, US

Assignee:

Gen-Probe Incorporated - San Diego CA

International Classification:

C12M 1/34
C12M 3/00
C07H 21/02
C07H 21/04
C12Q 1/68
C12P 19/34

US Classification:

4352871, 435 6, 435 912, 4352872, 536 231, 536 243

Abstract:

Compositions, methods and devices for detecting nucleic acids. The invention particularly regards composite arrays of immobilized amplification primers and hybridization probes. Also disclosed are compositions and methods for covalently immobilizing oligonucleotides and other biological molecules to glass and plastic surfaces.

US Patent:

8628924, Jan 14, 2014

Filed:

Jul 21, 2010

Appl. No.:

12/840971

Inventors:

Daniel L. Kacian - San Diego CA, US
Kenneth A. Browne - San Diego CA, US

Assignee:

Gen-Probe Incorporated - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 612, 435 912

Abstract:

Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e. g. , distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

US Patent:

20050202491, Sep 15, 2005

Filed:

Mar 4, 2005

Appl. No.:

11/073085

Inventors:

Norman Nelson - San Diego CA, US
Kenneth Browne - Poway CA, US
Lizhong Dai - San Diego CA, US
James Russell - Vista CA, US
Mark Filipowsky - San Marcos CA, US
Margarita Kaminsky - San Diego CA, US
Daniel Kacian - San Diego CA, US

International Classification:

C12Q001/68
A61K047/00

US Classification:

435006000, 424661000, 424126000

Abstract:

The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

US Patent:

20050287591, Dec 29, 2005

Filed:

Aug 24, 2005

Appl. No.:

11/211231

Inventors:

Kenneth Browne - Poway CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435006000, 435091200

Abstract:

Method of amplifying and detecting a nucleic acid target molecule contained in a test sample using a device having amplification primers and detectably labeled hybridization probes immobilized on the same surface of a solid support. Soluble amplicons produced in an in vitro nucleic acid amplification reaction employing the immobilized amplification primers are detected by the immobilized hybridization probes. Surface-immobilized amplification primers and hybridization probes are maintained in fluid communication throughout the procedure.

US Patent:

20060246500, Nov 2, 2006

Filed:

Jun 29, 2006

Appl. No.:

11/477988

Inventors:

Kenneth Browne - Poway CA, US

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

Method of detecting a target polynucleotide based on the use of dual inversion hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. The arms of the dual inversion hybridization probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the dual inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

US Patent:

20120231459, Sep 13, 2012

Filed:

Apr 12, 2012

Appl. No.:

13/445780

Inventors:

Kenneth A. BROWNE - Poway CA, US
Ian WEEKS - Cardiff, GB

Assignee:

GEN-PROBE INCORPORATED - San Diego CA

International Classification:

G01N 21/76
C12N 7/00
C07D 401/12
C07D 219/06
C07H 21/04
C08B 37/00
C07H 5/06
C12N 1/00
C07K 17/02

US Classification:

435 611, 436501, 435243, 4352351, 4353171, 546104, 530409, 530300, 536 231, 5361231, 530395, 536 187, 530396, 546103

Abstract:

A novel method is disclosed for simultaneous detection and quantification of two or more nucleic acid targets, without need for amplification. The method depends on spectral-temporal resolution of chemiluminescence emitted from independent hybridization-induced chemiluminescent signal (HICS) probes. The utility of this method has been demonstrated by use of resolvable N-linked acridinium and 2,7-dimethoxyacridinium ester labeled probes in a homogeneous assay for sensitive and simultaneous independent quantification of several bacterial and fungal target sequences. Compositions and kits for practicing the method of the present invention are also disclosed.