The present invention relates to an improved vessel for cell culture and methods for its use. The vessel of the present invention comprises a collapsible bag with an inner surface, an outer surface and a top periphery and a headplate having a circumferential edge wherein the top periphery of the bag is sealed to the edge of the headplate. The present invention also relates to an impeller for use with the vessel and a method for culturing cells.
The present invention relates to an improved vessel for cell culture and methods for its use. The vessel of the present invention comprises a collapsible bag with an inner surface, an outer surface and a top periphery and a headplate having a circumferential edge wherein the top periphery of the bag is sealed to the edge of the headplate. The present invention also relates to an impeller for use with the vessel and a method for culturing cells.
Brian Seibert - Monroeville NJ, US Kenneth B. Carll - Bridgeton NJ, US
Assignee:
Alcan Packaging Pharmaceutical & Personal Care Inc. - Millville NJ
International Classification:
C12M001/38 F27D011/00 F27D007/04
US Classification:
219400, 219386, 236 3
Abstract:
A cover for a rack to control the temperature of the contents of the rack is provided. The cover comprises a frame having a heater, a soft hood with a top and a bottom, the hood extending downward from the frame, and a duct fluidly connecting the heater to the bottom of the hood whereby the duct introduces heated air into the bottom of the hood. Also included is a method of controlling the temperature of a reaction using the cover of the present invention.
A circuit for measuring the conductivity of a solution by applying a known ac current source across a pair of electrodes in the solution and utilizing an analog-to-digital dual slope converter, such as those of the integrated circuit type which include capacitor sampling of the signal input, for conversion of the ac voltage drop across the electrodes. The circuit includes means for synchronizing the ac current to the clock to time the sampling so that the same polarity of signal input is always obtained. Where the known ac current source consists of switching the sense of the electrode connections to a combination of a dc source in series with a known resistor, used for supplying the reference input to the converter, a dc signal input, instead of ac, obtained by including the voltage drop of the switch with the drop across the electrodes in the signal sampled. With this arrangement, higher cell constants can be used to minimize the effect of the voltage drop across the switch.
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