Kenneth Paul Collier

US Patent:

47286091, Mar 1, 1988

Filed:

Sep 24, 1985

Appl. No.:

6/778779

Inventors:

Ram S. Bhatt - Nutley NJ
Kenneth J. Collier - Rockaway NJ
Robert M. Crowl - Little Falls NJ
Mohindar S. Poonian - West Caldwell NJ

Assignee:

Hoffmann-La-Roche Inc. - Nutley NJ

International Classification:

C12P 2100
C12P 2102
C12N 1500
C12N 120
C12N 100
C07H 1512

US Classification:

435 68

Abstract:

Th preparation of a double-stranded polydeoxy ribonucleotide having cohesive termini each comprising one strand of a double-strand restriction endonuclease recognition site and, between the termini, a structural gene coating for the expression of recombinant growth hormone releasing factor, at least a majority of the codons and the coding strand of said gene being codons preferred for the expression of microbial genomes; a method of producing such DNA a recombinant microbial cloning vehicle comprising such DNA and the novel methods of producing said peptide are described.