A clean connection and sampling apparatus (10) and method for creating a controlled environment for the transfer of chemicals has an isolation means for providing an isolated area (14) capable of being cleaned. A first connection means (16) for connection to a supply of chemicals to be transferred is provided and a second connection means (18) for transferring the chemicals from the isolation means to a receiver for said chemicals is also provided. A sampling means is located within the isolation means for sampling the chemical prior to transfer to the receiver. A pass-through means (22) is provided for receiving a sample from within the isolation means and isolating the sample from the isolation means prior to transfer of the sample outside of the isolation means for analysis. A gas purge means (26) is provided and connected to the isolation means for purging the isolation means with gas, preferably an inert gas such as nitrogen, and for forcing the chemical from the chemical supply through the isolation means to the receiver. A spray means (28) is also provided within the isolation means for operation by means of accordion gloves attached to accordion glove ports (34) to prevent contamination of the interior of the isolation means while enabling manipulation of the interior parts, such as stop valve (17), sampler (20) and spray means (28).
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