Kenneth Koo Koo

US Patent:

56959656, Dec 9, 1997

Filed:

Jun 2, 1992

Appl. No.:

7/896455

Inventors:

W. Dorsey Stuart - Kaneohe HI
John M. Ivy - Kailua HI
Kenneth Koo - Encino CA

Assignee:

Hawaii Biotechnology Group, Inc. - Aiea HI

International Classification:

C12P 2102

US Classification:

435 691

Abstract:

Neurospora crassa transformations are employed for production of heterologous proteins, where DNA constructs are prepared using a foreign gene under transcriptional and translational regulatory regions functional in Neurospora. A functional signal sequence is provided for secretion of the heterologous gene product. An auxotrophic Neurospora host is employed and transformed by the combination of the subject construct and a complementing gene. The resulting transformants provide for efficient secretion of the heterologous product.