Kevin D Chapman

US Patent:

20200392567, Dec 17, 2020

Filed:

Jun 24, 2020

Appl. No.:

16/910682

Inventors:

- Emeryville CA, US
Ravi K. Ramenani - Livermore CA, US
Debjit Ray - Santa Clara CA, US
Thomas M. Vetterli - Berkeley CA, US
Annamaria Mocciaro - San Francisco CA, US
Magali Soumillon - Boston MA, US
Mark P. White - Orinda CA, US
Troy A. Lionberger - Berkeley CA, US
Kevin T. Chapman - Emeryville CA, US
Po-Yuan Tung - South San Francisco CA, US

Assignee:

Berkeley Lights, Inc. - Emeryville CA

International Classification:

C12Q 1/6809
B01J 19/00
B01L 3/00
C12Q 1/6806
C12Q 1/6841

Abstract:

Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. The methods can include contacting a biological cell with a test agent for a period of time; lysing the biological cell while the biological cell is disposed within a sequestration pen located within an enclosure of a microfluidic device; and allowing RNA molecules released from the lysed biological cell to be captured by capture oligonucleotides linked to a capture object disposed within the sequestration pen of the microfluidic device. Each capture oligonucleotide can include a priming sequence that binds a primer, and a capture sequence. Each cDNA transcribed from a captured RNA can have an oligonucleotide sequence complementary to the captured RNA molecule, with the complementary oligonucleotide sequence being covalently linked to one of the capture oligonucleotides of the capture object.

US Patent:

20200064337, Feb 27, 2020

Filed:

Apr 22, 2019

Appl. No.:

16/391063

Inventors:

- Emeryville CA, US
Jason C. Briggs - Pleasanton CA, US
Jason M. McEwen - El Cerrito CA, US
Ravi K. Ramenani - Livermore CA, US
Hariharasudhan Chirra Dinakar - Fremont CA, US
Kai W. Szeto - Berkeley CA, US
Adrienne T. Higa - San Francisco CA, US
Mark P. White - Orinda CA, US
Xiaohua Wang - Ho Ho Kus NJ, US
Kevin T. Chapman - Emeryville CA, US

International Classification:

G01N 33/50
B01L 3/00
C12N 5/0781
G01N 33/68
C12Q 1/6876

Abstract:

Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.