Kevin G O'Malley

US Patent:

20080085528, Apr 10, 2008

Filed:

Oct 10, 2007

Appl. No.:

11/869778

Inventors:

Jovita Marcinkeviciene - Washington Crossing PA, US
Kevin O'Malley - Chalfont PA, US
Mark Kirby - New Hope PA, US
Aiying Wang - West Windsor NJ, US
Lisa Kopcho - Pemberton Borough NJ, US
Ramakrishna Seethala - West Windsor NJ, US

International Classification:

C12Q 1/48
C12Q 1/54

US Classification:

435014000, 435015000

Abstract:

The subject matter disclosed and claimed herein relates to novel in vitro assays for measuring glucokinase activity and use of these assays for identifying modulators of glucokinase.

US Patent:

20100311072, Dec 9, 2010

Filed:

Jan 28, 2009

Appl. No.:

12/864745

Inventors:

Ramakrishna Seethala - West Windsor NJ, US
Jessica Leihwa Dong - Brisbane CA, US
Kevin M. O'Malley - Chalfont PA, US

International Classification:

G01N 33/53
C12Q 1/68

US Classification:

435 6, 435 78, 435 76

Abstract:

The subject matter disclosed and claimed herein concerns measuring the binding affinity of glucokinase (“GK”) using a fluorescence polarization (“FP”) assay. The FP method includes use of modified GK ligands bound to a fluorescent label. Binding affinity is determined by measuring displacement of fluorescent ligand by the known or suspected GK ligands. The subject matter disclosed and claimed herein provides a robust high-throughput FP assay for the determination of binding affinity of ligands to glucokinase. The FP binding assay displayed both glucose and nucleotide dependence, and a useful dynamic range. The binding ICdata correlated well with GK activation ECdata.