Kevin Scott Roth

US Patent:

6372937, Apr 16, 2002

Filed:

Nov 5, 1999

Appl. No.:

09/434742

Inventors:

Mark Norman Bobrow - Lexington MA 02421
Karl Edwin Adler - Newburyport MA 01950
Kevin Aaron Roth - Webster Groves MO 63119

International Classification:

C07C22900

US Classification:

562444, 564167

Abstract:

A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent conmprising an inorganic salt, an organic compound having the formula wherein when X is B(OH) , Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from âOH, âCOOH, âNH , and âSH, and wherein R is selected from âOH, âCOOH, âNH , and âCH ; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R is âCH OH, âCOOH, and n is 1-8.

US Patent:

6518036, Feb 11, 2003

Filed:

Mar 16, 2000

Appl. No.:

09/526414

Inventors:

Mark Norman Bobrow - Lexington MA
Kevin Aaron Roth - Webster Groves MO

Assignee:

Nen Life Science Products, Inc. - Boston MA
Washington University - St. Louis MO

International Classification:

C12Q 128

US Classification:

435 28, 562450, 564170

Abstract:

A method for performing a peroxidase-based assay by reacting a peroxidase enzyme with a soluble organic p-hydroxyphenyl-containing compound having the formula: wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from âOH, âCOOH, âNH , and âSH, and wherein R is selected from âOH, âCOOH, âNH , and âCH ; and converting the soluble organic p-hydroxyphenyl-containing compound into a stable insoluble product which becomes highly fluorescent after illumination, upon excitation.

US Patent:

6593100, Jul 15, 2003

Filed:

May 17, 2001

Appl. No.:

09/859988

Inventors:

Mark Norman Bobrow - Lexington MA
Karl Edwin Adler - Newburyport MA
Kevin Aaron Roth - Webster Groves MO

Assignee:

NEN Life Science Products, Inc. - Boston MA
Washington University - St. Louis MO

International Classification:

C12Q 128

US Classification:

435 28, 562444

Abstract:

A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent comprising an inorganic salt, an organic compound having the formula wherein when X is B(OH) , Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein tie heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from âOH, âCOOH, âNH , and âSH, and wherein R is selected from âOH, âCOOH, âNH , and âCH ; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R is âCH OH, âCOOH, and n is 1-8.