Kimberly S Lamb

US Patent:

6485648, Nov 26, 2002

Filed:

Oct 25, 2000

Appl. No.:

09/696610

Inventors:

Douglas T. Gjerde - Saratoga CA
Robert M. Haefele - Campbell CA
Laura Bunnel - Omaha NE
Paul D. Taylor - Gilroy CA
Kimberly A. Lamb - Omaha NE
Satyajit Kar - Omaha NE

Assignee:

Transgenomic, Inc. - San Jose CA

International Classification:

B01D 1508

US Classification:

210635, 210656, 210659, 2101982

Abstract:

An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit. A process for cleaning the non-polar DNA separation surfaces in the apparatus comprising interrupting the flow of separation solvent with a block of cleaning solution injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from the non-polar surface. The cleaning solution can have an alkaline pH and contain a chelating agent such as EDTA.

US Patent:

6136195, Oct 24, 2000

Filed:

Apr 2, 1999

Appl. No.:

9/285331

Inventors:

Paul D. Taylor - Palo Alto CA
Robert M. Haefele - Palo Alto CA
Douglas T. Gjerde - Saratoga CA
Kimberly A. Lamb - Omaha NE

Assignee:

Transgenomic, Inc. - San Jose CA

International Classification:

B01D 1508

US Classification:

210635

Abstract:

An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit. A process for cleaning the non-polar DNA separation surfaces in the apparatus comprising interrupting the flow of separation solvent with a block of cleaning solution injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from the non-polar surface. The cleaning solution can have an alkaline pH and contain a chelating agent such as EDTA.

US Patent:

6218153, Apr 17, 2001

Filed:

May 13, 1999

Appl. No.:

9/311116

Inventors:

Jeffrey L. Sklar - Chestnut Hill MA
Douglas T. Gjerde - Saratoga CA
Kimberly A. Lamb - Omaha NE
Christopher Hanna - Somerville MA

Assignee:

Transgenomic, Inc. - San Jose CA

International Classification:

C12Q 168
C12P 1934
C07H 2104
B01D 1500

US Classification:

435 912

Abstract:

A method for detecting a putative mutant DNA in a sample of DNA includes the steps of amplifying the sample of DNA using PCR; hybridizing the amplified sample to form a mixture of homoduplexes and heteroduplexes; separating the mixture into fractions by Denaturing Matched Ion Polynucleotide Chromatography; and blind collecting the eluted fractions at a retention time corresponding to the retention time of the heteroduplex. The DNA in the blind collected fractions can be PCR amplified to obtain an increased amount of heteroduplex relative to homoduplex. The method is useful for determining the remission status of a patient in which the tissue-derived DNA sample contains a large background of wild type or where the putative mutant DNA is below the limit of detection.