Kimberly J Nichols

US Patent:

20070173476, Jul 26, 2007

Filed:

Jan 4, 2007

Appl. No.:

11/619993

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US
Yurity Fedorov - Superior CO, US
Kimberly Nichols - Louisville CO, US

Assignee:

DHARMACON INC. - Lafayette CO

International Classification:

A61K 48/00
C07H 21/02
C07J 17/00

US Classification:

514044000, 514026000, 514081000, 536023100, 536006100

Abstract:

Methods and compositions for performing RNA interference comprising a wide variety of stabilized siRNAs suitable for use in serum-containing media and for in vivo applications, such as therapeutic applications, are provided. These siRNAs permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of one or more modifications including orthoesters, terminal conjugates, modified linkages and 2′modified nucleotides. Uniquely modified siRNAs have been developed that reduces off-target effects incurred in gene-silencing. The modifications include phosphorylation of the first 5′ terminal antisense nucleotide; 2′ carbon modifications of the first and second or first, second, and third 5′ terminal antisense nucleotides; and optionally 2′ carbon modifications of the first and second or first, second, and third 5′ terminal sense nucleotide. Control and exaequo molecules are also provided. siRNA molecules and related control, trackability and exaequo agents with specific stability modifications were developed.

US Patent:

20100197023, Aug 5, 2010

Filed:

Nov 25, 2009

Appl. No.:

12/626011

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US
Yuriy Fedorov - Superior CO, US
Kimberly Nichols - Louisville CO, US

Assignee:

DHARMACON INC. - Lafayette CO

International Classification:

C12N 15/87
C07H 21/04

US Classification:

435463, 536 245

Abstract:

Methods and compositions for performing RNA interference comprising a wide variety of stabilized siRNAs suitable for use in serum-containing media and for in vivo applications, such as therapeutic applications, are provided. These siRNAs permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of one or more modifications including orthoesters, terminal conjugates, modified linkages and 2′ modified nucleotides. Uniquely modified siRNAs have been developed that reduces off-target effects incurred in gene-silencing. The modifications include phosphorylation of the first 5′ terminal antisense nucleotide; 2′ carbon modifications of the first and second or first, second, and third 5′ terminal antisense nucleotides; and optionally 2′ carbon modifications of the first and second or first, second, and third 5′ terminal sense nucleotide. Control and exaequo molecules are also provided. siRNA molecules and related control, trackability and exaequo agents with specific stability modifications were developed.

US Patent:

20070167384, Jul 19, 2007

Filed:

Apr 1, 2004

Appl. No.:

10/551350

Inventors:

Devin Leake - Denver CO, US
Angela Reynolds - Conifer CO, US
Anastasia Khvorova - Boulder CO, US
William Marshall - Boulder CO, US
Yuriy Fedorov - Superior CO, US
Kimberly Nichols - Louisville CO, US

Assignee:

DHARMACON, INC. - Lafayette CO

International Classification:

A61K 48/00
C07J 17/00
C07H 21/02

US Classification:

514044000, 514026000, 536006100, 536023100

Abstract:

p-00010000