Lawrence Dr Restaino

US Patent:

6416970, Jul 9, 2002

Filed:

Sep 19, 2000

Appl. No.:

09/646528

Inventors:

Günter Schabert - Goldach, CH
Lawrence Restaino - Chicago IL

Assignee:

Biosynth AG - Staad

International Classification:

C12Q 104

US Classification:

435 34, 435 4, 435968, 435975

Abstract:

The invention provides compounds of formula (I) in which R , R , R , R and R are independently selected from hydrogen and chromogenic substituents, and X is selected from the group consisting of hydroxyl; OR wherein R is selected from the group consisting of C -C alkyl; and O Me wherein Me is a cation derived from an organic or inorganic base. A preferred species of formula (I) is 4-Methylumbelliferyl myo-inositol-1-phosphate and salts thereof with an organic or inorganic base. The invention also provides fluorogenic methods for detecting various pathogenic bacteria, e. g. , Listeria, Staphylococcus and Clostridium species, using substrates containing at least one formula (I) compound. A kit for detecting a phosphatidyl-inositol-specific phospholipase C enzyme as an indication of bacterial activity is disclosed.

US Patent:

6617149, Sep 9, 2003

Filed:

Mar 18, 2002

Appl. No.:

10/100261

Inventors:

Lawrence Restaino - Elburn IL

Assignee:

RF Laboratories, Inc. - West Chicago IL

International Classification:

C12N 120

US Classification:

4352528, 435 34, 435 38, 435 39, 435 40, 4352536, 4352879

Abstract:

An isolation plating medium for use in processes for the presumptive identification of 0157:H7 from a sample that also contains other strains of The plating medium comprises at least one carbohydrate that 0157:H7 is incapable of fermenting, but other strains of do ferment said carbohydrate, a pH indicator dye that changes the color of the plating medium to a first color when the pH of the medium changes, a chromogenic substrate that reacts to beta-galactosidase to form a precipitate in the plating medium of a second color which contrasts with the first color. Both 0157:H7 and the other strains of are beta-galactosidase producers during metabolism, whereby a microorganism which ferments the carbohydrate but does not produce beta-galactosidase will produce colonies in the plating medium of the first color, 0157:H7 which does not ferment the carbohydrate but produces beta-galactosidase will produce colonies in the plating medium of the second color, and the other strains of which ferment the carbohydrate medium and produce beta-galactosidase will produce colonies in the plating medium of a third color which is the color that results from the mixing of the first and second colors, and a sufficient mass of an agent to solidify the mixture. The invention also includes processes of using the plating medium to identify and enumerate 0157:H7 and other microorganisms.

US Patent:

6660494, Dec 9, 2003

Filed:

Dec 19, 2001

Appl. No.:

10/021271

Inventors:

Günter Schabert - Goldach, CH
Lawrence Restaino - Chicago IL
Elon W. Frampton - DeKalb IL

Assignee:

Biosynth AG

International Classification:

C12Q 104

US Classification:

435 34, 435 29, 435 18, 435 21, 435 32, 43525231, 4352533, 435975, 548414

Abstract:

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I): wherein R is selected from the group consisting of hydrogen and C alkyl, such as methyl, ethyl, propyl and butyl while R , R , R , and R are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C alkyl groups, aminomethyl, hydroxy, C alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.

US Patent:

6764832, Jul 20, 2004

Filed:

Aug 22, 2001

Appl. No.:

09/934506

Inventors:

Lawrence Restaino - Elburn IL 60119

International Classification:

C12Q 104

US Classification:

435 34, 435968, 435 39, 435 38, 435 40, 435 29, 435973

Abstract:

A solid growth plating medium in which Shigella organisms will grow and form colonies in the medium, and substantially, other microorganisms are inhibited or their colonies are differentiated from Shigella organisms. In one embodiment of the invention, colonies produced by Shigella appear with the color of the plating medium, usually a clear off-white color, that can be readily observed. In another embodiment, the fact that and produce the enzyme alpha-galactosidase, but most and strains do not, is utilized with a chromogenic substrate to produce colonies of these microorganisms of a distinguishing color.

US Patent:

7150977, Dec 19, 2006

Filed:

Feb 23, 2004

Appl. No.:

10/784347

Inventors:

Lawrence Restaino - Elburn IL, US

Assignee:

R&F Products, Inc. - Downers Grove IL

International Classification:

C12Q 1/10
C12Q 1/04
C12Q 1/54
C12Q 1/34

US Classification:

435 38, 435 34, 435 14, 435 18

Abstract:

An isolation plating medium for the identification of bacteria in a sample containing a plurality of different bacteria comprising a mixture of a carbohydrate capable of being a metabolic source for bacteria and supporting colonies of bacteria, a pH indicator dye that changes the color of the plating medium to a first color different from the color of the medium responsive to a change in the pH of the medium, a first substrate that does not react with bacteria and injects color into the medium of a second color responsive to the presence of beta-galactosidase, the second color contrasting with the first color and the color of the medium, a second substrate that does not react with bacteria and injects color into the medium of substantially the same color as the second color responsive to the presence of beta=galactosidase, and an ingredient for thickening the mixture in sufficient quantity to solidify the mixture.

US Patent:

7309580, Dec 18, 2007

Filed:

Jul 5, 2002

Appl. No.:

10/188552

Inventors:

Lawrence Restaino - Elburn IL, US

Assignee:

R&F Products, Inc. - Downers Grove IL

International Classification:

C12Q 1/04
C12N 1/20

US Classification:

435 34, 4352536

Abstract:

A plating medium for the presumptive identification of and/or that includes a nutrient base to facilitate growth of and , a chromogenic substrate that changes color responsive to the presence of PC-PLC enzymes, and an ingredient that promotes the expression of the PC-PLC enzyme. In a preferred embodiment, the medium includes a second chromogenic substrate that changes color responsive to the presence of PI-PLC enzymes and an ingredient that promotes the expression of the PI-PLC enzyme.

US Patent:

20060257967, Nov 16, 2006

Filed:

May 13, 2005

Appl. No.:

11/128741

Inventors:

Lawrence Restaino - Elburn IL, US

International Classification:

C12Q 1/04
C12Q 1/16

US Classification:

435034000, 435035000

Abstract:

A plating medium for identification of bacteria having a carbohydrate, but bacteria being incapable of fermenting any carbohydrate in the medium. The medium also contains a pH indicator dye that changes the color of the medium from a first color to a second color when the pH changes, first and second chromogenic substrates that react to alpha-glucosidase and beta cellobiosidase enzymes, respectively, to produce a third color in the medium, and agar to solidify the mixture. Microorganisms that ferment the carbohydrate but do not produce alpha-glucosidase or beta-cellobiosidase will produce colonies of the second color, microorganisms that produce alpha-glucosidase and/or beta-cellobiosidase including bacteria will produce colonies of the third color, and microorganisms that ferment the carbohydrate and produce alpha glucosidase and/or beta-cellobiosidase will produce colonies of a fourth color which is the color that results from mixing the second and third colors.

US Patent:

20070004021, Jan 4, 2007

Filed:

Jun 28, 2005

Appl. No.:

11/168830

Inventors:

Lawrence Restaino - Elburn IL, US

International Classification:

C12N 1/20

US Classification:

435253600

Abstract:

A plating media for identifying bacteria having nutrients for promoting growth of thereby producing beta-glucosidase, carbohydrate that is incapable of reacting with a pH indicator dye, a chromogenic substrate that reacts to beta-glucosidase to form precipitate, and an agent to solidify the mixture, whereby a microorganism which ferments the carbohydrate but does not produce beta-glucosidase will produce colonies of the color determined by the pH indicator dye, and other microorganisms that do not ferment the carbohydrate but produce beta-glucosidase activate the substrate to color their colonies with the color of precipitate released by the substrate, and other bacteria which ferment the carbohydrate and produce beta-glucosidase produce colonies of the color that results from mixing the colors described above.