Leslie G Bell

US Patent:

58719571, Feb 16, 1999

Filed:

Aug 22, 1994

Appl. No.:

8/293568

Inventors:

Glenn H. Kawasaki - Seattle WA
Leslie Bell - Princeton NJ

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12P 2102
C12N 115

US Classification:

435 691

Abstract:

Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is s strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.

US Patent:

49313738, Jun 5, 1990

Filed:

Oct 22, 1984

Appl. No.:

6/663315

Inventors:

Glenn Kawasaki - Seattle WA
Leslie Bell - Seattle WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12P 2100
C12P 2102
C12N 1500
C12N 700

US Classification:

435 692

Abstract:

Methods are provided for producing. alpha. -1-antitrypsin in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selectable marker whereby the growth conditions for selection may comprise a conventional complex medium.

US Patent:

55276687, Jun 18, 1996

Filed:

Oct 12, 1993

Appl. No.:

8/134480

Inventors:

Glenn Kawasaki - Seattle WA
Leslie Bell - Princeton NJ

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12Q 168
C12P 2102
C12N 119
C12N 1581

US Classification:

435 6

Abstract:

Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.

US Patent:

57232920, Mar 3, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/488179

Inventors:

Glenn H. Kawasaki - Seattle WA
Leslie Bell - Princeton NJ
Meher Irani - Seattle WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12P 2102

US Classification:

435 6

Abstract:

Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is s strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.