Leslie P Oppenheimer

US Patent:

53995007, Mar 21, 1995

Filed:

Jun 26, 1992

Appl. No.:

7/906213

Inventors:

Leslie Oppenheimer - Kinnelon NJ
Lewis R. Pollack - Riverdale NY

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 33543
G01N 33545

US Classification:

436500

Abstract:

A process for coating an antibody on a substrate includes two steps. A secondary antibody to a primary antibody is coated on the substrate followed by a coating with primary antibody in the presence of blocking and stabilizer agents.

US Patent:

52485900, Sep 28, 1993

Filed:

Jul 22, 1991

Appl. No.:

7/333937

Inventors:

Herman Rutner - Hackensack NJ
Josephine D. Readio - Sparta NJ
Leslie Oppenheimer - Kinnelon NJ

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12Q 128
G01N 33544
G01N 33547

US Classification:

435 5

Abstract:

A liposome reagent encapsulating a molecule to be targeted to a body site or used as an assay reporter has a ligand and a sulfonate-containing group on the liposome surface. Preferred ligands are antibodies or antibody fragments and preferred encapsulants are enzymes or dyes. In the most preferred reagents, the antibody and sulfonate-containing group are covalently bonded to the liposome surface through a connecting group which includes a succinimidyl group resulting from addition of the ligand or sulfonate-containing group to a maleimidyl group. The invention includes a kit of materials for performing an assay using the reagent of the invention as the tracer.