Li I Li

US Patent:

7085716, Aug 1, 2006

Filed:

Oct 26, 2000

Appl. No.:

09/696685

Inventors:

Stijn Van Even - Jamaica Plain MA, US
Li Li - Bedminster NJ, US
Xianju Du - Acton MA, US
Puming Zhan - Acton MA, US

Assignee:

Nuance Communications, Inc. - Burlington MA

International Classification:

G10I 15/26

US Classification:

704235, 704251

Abstract:

A method is described that corrects incorrect text associated with recognition errors in computer-implemented speech recognition. The method includes the step of performing speech recognition on an utterance to produce a recognition result for the utterance. The command includes a word and a phrase. The method includes determining if a word closely corresponds to a portion of the phrase. A speech recognition result is produced if the word closely corresponds to a portion of the phrase.

US Patent:

20060115472, Jun 1, 2006

Filed:

Aug 12, 2005

Appl. No.:

11/203367

Inventors:

Li Li - Sudbury MA, US
Angela Kantor - Pepperell MA, US
Shannon MacMillan - Hollis NH, US

Assignee:

Wyeth - Madison NJ

International Classification:

A61K 39/395

US Classification:

424133100

Abstract:

Formulations are described that are suitable for storage of proteins, such as antibodies, over a relatively broad range of protein concentrations, pH, and buffer types. Also described are methods of storing a protein and methods of identifying a suitable formulation for storage of a specific protein. In general, a formulation contains low to no surfactant, no to relatively low salt concentrations, and requires a relatively low buffer concentration.

US Patent:

20060166251, Jul 27, 2006

Filed:

Jan 26, 2006

Appl. No.:

11/339549

Inventors:

Joanne Archambault - Medford MA, US
Scott Jelinsky - Waltham MA, US
Michael Agostino - Andover MA, US
Li Li - Watertown MA, US
Howard Seeherman - Cambridge MA, US

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

The disclosure provides assay systems for evaluating the presence of bone morphogenetic protein (BMP) activity in a cell by evaluating the gene expression of secreted Frizzled Related Protein 2 and 3 (sFRP2 and sFRP3). The sFRP2 and sFRP3 gene expression may be detected at the RNA or protein levels. The methods include methods for evaluating exogenous and endogenous BMP expression and utilize both genomic sFRP2 and sFRP3 genes and reporter constructs.

US Patent:

20070237758, Oct 11, 2007

Filed:

Nov 21, 2006

Appl. No.:

11/562299

Inventors:

Anthony Barry - Winchester MA, US
Thomas Crowley - Wilmington MA, US
Daniel Dixon - Arlington MA, US
Jennifer Juneau - Sterling MA, US
Ajay Kumar - North Andover MA, US
Li Li - Sudbury MA, US
Nicholas Luksha - Malden MA, US
Michael Shamashkin - Somerville MA, US
Erin Soley - Lowell MA, US
Nicholas Warne - Andover MA, US
Chandra Webb - Pelham NH, US

International Classification:

A61K 39/395

US Classification:

424130100

Abstract:

The present invention provides compositions of Ig fusion proteins, especially compositions including an Ig fusion protein, a bulking agent, a disaccharide, a surfactant, and a buffer. In one aspect, these compositions are stable under long-term storage or at least one freeze/thaw cycle. The invention also provides methods of preparation of the Ig fusion protein compositions. In one aspect, compositions of the invention are lyophilized. In a further aspect, the compositions are lyophilized by a process that includes an annealing step.

US Patent:

20080003686, Jan 3, 2008

Filed:

May 17, 2007

Appl. No.:

11/804191

Inventors:

Li Li - Sudbury MA, US
Angela Kantor - Pepperell MA, US
Nicholas Warne - Andover MA, US

Assignee:

Wyeth - Madison NJ

International Classification:

G01N 33/00

US Classification:

436086000

Abstract:

A method is described for predicting the relative solubility of a polypeptide using polyethylene glycol (PEG) based volume exclusion precipitation. Different polypeptides can be tested for their solubilities relative to each other or relative to a reference. A single polypeptide can be tested for its relative solubility under different experimental conditions. The solubility determinations can be made by comparison based on graphs plotting the log solubility of the polypeptide against a range of PEG concentrations. Additionally, a method is provided for the high throughput visual or automated screening of multiple polypeptides for relative solubility differences, in a method that can omit the step of measuring the actual solubility or actual amount of precipitation of each sample at each PEG concentration.

US Patent:

20080064856, Mar 13, 2008

Filed:

Mar 19, 2007

Appl. No.:

11/725534

Inventors:

Nicholas Warne - Andover MA, US
Angela Kantor - Pepperell MA, US
Thomas Crowley - Wilmington MA, US
Erin Soley - Lowell MA, US
Li Li - Sudbury MA, US
Nicholas Luksha - Malden MA, US
Edie Neidhardt - Boxford MA, US

Assignee:

Wyeth - Madison NJ

International Classification:

C07K 14/755
C07K 1/14
C07K 14/00

US Classification:

530383000, 530350000, 530412000

Abstract:

Methods of reducing aggregation of a protein or proteins in a formulation, and protein formulations having reduced aggregation properties are provided. The methods and formulations described herein maintain the biological activity of a protein and increase the shelf life of protein formulations.

US Patent:

20080070230, Mar 20, 2008

Filed:

Jun 11, 2007

Appl. No.:

11/811517

Inventors:

Li Li - Sudbury MA, US
Angela Kantor - Pepperell MA, US
Nicholas Warne - Andover MA, US

Assignee:

Wyeth - Madison NJ

International Classification:

G01N 33/536
C07K 1/00
G01N 33/00

US Classification:

435004000, 436536000, 530412000

Abstract:

Methods of formulating proteins at high concentrations in protein solutions, wherein the protein solutions lack or have reduced liquid-liquid phase separation are described. Such protein solutions are substantially clear and substantially homogenous. Additionally, methods of concentrating and purifying proteins using liquid-liquid phase separation are provided.

US Patent:

20110033464, Feb 10, 2011

Filed:

Oct 22, 2010

Appl. No.:

12/910146

Inventors:

Anthony BARRY - Winchester MA, US
Thomas Crowley - Wilmington MA, US
Daniel Dixon - Arlington MA, US
Jennifer Juneau - Sterling MA, US
Ajay Kumar - North Andover MA, US
Li Li - Sudbury MA, US
Nicholas Luksha - Malden MA, US
Michael Shamashkin - Somerville MA, US
Erin Soley - Lowell MA, US
Nicholas Warne - Andover MA, US
Chandra Webb - Pelham NH, US

International Classification:

A61K 39/395

US Classification:

4241341

Abstract:

The present invention provides compositions of Ig fusion proteins, especially compositions including an Ig fusion protein, a bulking agent, a disaccharide, a surfactant, and a buffer. In one aspect, these compositions are stable under long-term storage or at least one freeze/thaw cycle. The invention also provides methods of preparation of the Ig fusion protein compositions. In one aspect, compositions of the invention are lyophilized. In a further aspect, the compositions are lyophilized by a process that includes an annealing step.