Li Yan Zhu

US Patent:

6406863, Jun 18, 2002

Filed:

Jun 23, 2000

Appl. No.:

09/603663

Inventors:

Li Zhu - Palo Alto CA
Shaobing Benjamin Hua - Cupertino CA

Assignee:

GeneTastix Corporation - San Jose CA

International Classification:

G01N 3353

US Classification:

435 71, 435 78, 435 29, 435 697, 435 711

Abstract:

Compostions, kits and methods are provided for generating highly diverse libraries of proteins such as antibodies via homologous recombination in vivo, and screening these libraries against protein, peptide and nucleic acid targets using a two-hybrid method in yeast. The method for screening a library of tester proteins against a target protein or peptide comprises: expressing a library of tester proteins in yeast cells, each tester protein being a fusion protein comprised of a first polypeptide subunit whose sequence varies within the library, a second polypeptide subunit whose sequence varies within the library independently of the first polypeptide, and a linker peptide which links the first and second polypeptide subunits; expressing one or more target fusion proteins in the yeast cells expressing the tester proteins, each of the target fusion proteins comprising a target peptide or protein; and selecting those yeast cells in which a reporter gene is expressed, the expression of the reporter gene being activated by binding of the tester fusion protein to the target fusion protein.

US Patent:

6410246, Jun 25, 2002

Filed:

Jun 23, 2000

Appl. No.:

09/603658

Inventors:

Li Zhu - Palo Alto CA
Shaobing Benjamin Hua - Cupertino CA

Assignee:

Genetastix Corporation - San Jose CA

International Classification:

G01N 3353

US Classification:

435 71, 435 731, 4353201, 536 234, 536 2353, 935 28

Abstract:

A highly diverse library of yeast expression vectors encoding a library of fusion proteins such as antibodies is provided. The yeast expression vector formed in the library comprises: a first nucleotide sequence encoding a first polypeptide subunit; a second nucleotide sequence encoding a second polypeptide subunit; and a linker sequence encoding a linker peptide that links the first nucleotide sequence and the second nucleotide sequence. The first polypeptide subunit, the second polypeptide subunit, and the linker polypeptide are expressed as a single fusion protein within the library of fusion proteins. The first and second nucleotide sequences each independently varies within the library of expression vectors. The library of fusion proteins expressed by the library expression vectors can be used for screening against target molecules such as proteins, peptides, DNAs and small molecules in vitro and in vivo.

US Patent:

6410271, Jun 25, 2002

Filed:

Jun 23, 2000

Appl. No.:

09/602373

Inventors:

Li Zhu - Palo Alto CA
Shaobing Benjamin Hua - Cupertino CA

Assignee:

GeneTasTix Corporation - San Jose CA

International Classification:

C12P 2104

US Classification:

435 697, 435 71, 435 691, 435 711, 435483

Abstract:

Methods are provided for generating highly diverse libraries of expression vectors encoding fusion proteins such as single-chain antibodies via homologous recombination in yeast. The method comprises: transforming into yeast cells a linearized yeast expression vector having a 5â- and 3â-terminus sequence at the site of linearization and a library of insert nucleotide sequences that are linear and double-stranded; and having homologous recombination occur between the vector and the insert sequence such that the insert sequence is included in the vector in the transformed yeast cells. The insert sequence comprises a first nucleotide sequence encoding a first polypeptide subunit, a second nucleotide sequence encoding a second polypeptide subunit, a linker sequence encoding a linker peptide that links the first and second polypeptide subunits, and a 5â- and 3â-flanking sequence at the ends of the insert sequence which are sufficiently homologous to the 5â- and 3â-terminus sequences of the linearized yeast expression vector, respectively, to enable homologous recombination to occur. The first polypeptide subunit, the second polypeptide subunit, and the linker polypeptide are expressed as a single fusion protein; and the first and second nucleotide sequences each independently varies within the library of expression vectors.

US Patent:

6610472, Aug 26, 2003

Filed:

Oct 31, 2000

Appl. No.:

09/703399

Inventors:

Li Zhu - Palo Alto CA
Shaobing Benjamin Hua - Cupertino CA
James Sheridan - Mountain View CA
Yu-Huei Lin - Sunnyvale CA

Assignee:

Genetastix Corporation - San Jose CA

International Classification:

G01N 3353

US Classification:

435 5, 435 731, 435DIG 47, 435 71, 935 28

Abstract:

Compositions, methods, and kits are provided for efficiently generating and screening a library of highly diverse protein complexes for their ability to bind to other proteins or oligonucleotide sequences. In one aspect of the invention, a library of expression vectors is provided for expressing the library of protein complexes. The library comprises a first nucleotide sequence encoding a first polypeptide subunit; and a second nucleotide sequence encoding a second polypeptide subunit. The first and second nucleotide sequences each independently varies within the library of expression vectors. In addition, the first and second polypeptide subunit are expressed as separate proteins which self-assemble to form a protein complex, such as a double-chain antibody fragment (dcFv or Fab) and a fully assembled antibody, in cells into which the library of expression vectors are introduced. The library of expression vectors can be efficiently generated in yeast cells through homologous recombination; and the encoded proteins complexes with high binding affinity to their target molecule can be selected by high throughput screening in vivo or in vitro.

US Patent:

7005503, Feb 28, 2006

Filed:

Feb 8, 2002

Appl. No.:

10/072301

Inventors:

Shaobing Hua - Cupertino CA, US
Michelle H. Pauling - San Mateo CA, US
Li Zhu - Palo Alto CA, US

Assignee:

Genetastix Corporation - San Jose CA

International Classification:

C07K 16/00

US Classification:

5303871, 5303873, 53038815, 53038873, 53038875, 435 7021, 435455, 435326, 435328, 435334, 4353431, 4353201, 4241331, 4241351, 4241391, 4241411, 4241421, 4241431

Abstract:

Compositions are provided that comprise antibody against coreceptors for human immunodeficiency virus such as CCR5 and CXCR4. In particular, monoclonal human antibodies against human CCR5 are provided that bind to CCR5 with high affinity and are capable of inhibiting HIV infection at low concentrations. The antibodies can be used as prophylactics or therapeutics to prevent and treat HIV infection, for screening drugs, and for diagnosing diseases or conditions associated with interactions with HIV coreceptors.

US Patent:

7138496, Nov 21, 2006

Filed:

Feb 7, 2003

Appl. No.:

10/360828

Inventors:

Shaobing Hua - Cupertino CA, US
Michelle Haynes Pauling - San Mateo CA, US
Li Zhu - Palo Alto CA, US

Assignee:

Genetastix Corporation - San Jose CA

International Classification:

C07K 16/00
C12P 21/08

US Classification:

5303871, 5303873, 5303879, 5303881, 5303882, 53038822, 5303887, 5303888, 5303897

Abstract:

Compositions are provided that comprise antibody against membrane proteins such as chemokine receptors. In particular, monoclonal human antibodies against human CXCR4 are provided that are capable of inhibiting HIV infection and chemotaxis in human breast cancer cells. The antibodies can be used as prophylactics or therapeutics to prevent and treat HIV infection and cancer, for screening drugs, and for diagnosing diseases or conditions associated with interactions with chemokine receptors.

US Patent:

7554773, Jun 30, 2009

Filed:

Mar 16, 2005

Appl. No.:

11/080657

Inventors:

Li Yan Zhu - Milpitas CA, US
Ming Gao Yao - GuangDong, CN
Masashi Shiraishi - Hong Kong, CN

Assignee:

SAE Magnetics (H.K.) Ltd. - Hong Kong

International Classification:

G11B 5/58

US Classification:

3602944

Abstract:

A HGA of the invention includes a slider; a micro-actuator to adjust the position of the slider; and a suspension to load the slider and the micro-actuator. The micro-actuator includes two side arms; a load plate for supporting the slider, which is connected with at least one of the side arms; a piezoelectric element connected with the side arms; and a base shaft to couple with the suspension, which is connected with the side arms and positioned between the load plate and the piezoelectric element. The invention also discloses a disk drive unit using such a HGA.

US Patent:

7700302, Apr 20, 2010

Filed:

Nov 6, 2006

Appl. No.:

11/593957

Inventors:

Shaobing Hua - Cupertino CA, US
Li Zhu - Palo Alto CA, US

Assignee:

Adimab, Inc. - Lebanon NH

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

Methods and compositions are provided for efficient, high throughput screening of antibody libraries against proteins targets, especially membrane proteins in yeast. In particular, methods are provided for screening a diverse antibody library for antibodies that bind to a membrane protein such as a chemokine receptor in yeast.