Department of Neuroscience, Georgetown University Medical Center
2013 to 2000 Instructor, Research TrackLaboratory of Molecular Pharmacology, National Cancer Institute, NIH Bethesda, MD Jan 2007 to Oct 2011 Postdoctoral FellowDepartment of Biological Sciences & MCB program, Ohio University. Athens, OH 2002 to 2006 Graduate Research Assistant
Education:
Ohio University Athens, OH 2006 PhD in Biological Sciences & Molecular and Cellular BiologyCollege of Life Sciences, Wuhan University Wuhan, China 2000 BS in Biological Division (Molecular, Cellular and Developmental Biology)
Skills:
Molecular Cellular Biology and Biochemistry: Expert in a wide range of molecular, cellular and biochemical techniques (Selected skills listed blow): Establish stable cell lines Gel electrophoresis: DNA, RNA, Sequencing & analysis software Lentivirus production Protein (including native gel) Affinity purification rAAV production Dual-Luciferase assay Nascent strand DNA replication Molecular cloning IP: Immunoprecipitation initiation analysis DNA, RNA isolation Western blot Cell elutriation centrifugation Quantitative PCR, iPCR Biotin-pull down Replication timing analysis Bacteria transformation FPLC Bisulfite genomic sequencing Transduction; transfection Immunofluorescence microscopy (DNA methylation analysis) Site-Directed Mutagenesis Cell suspension preparation Dnmt1 assay cDNA synthesis Mammalian cell culture Cell marker analysis with flow In vitro transcription Drosophila S2 cell culture cytometry; FACS DNA & cRNA labeling Cell transfection and selection Cell proliferation assays Microarray analysis Recombinase-mediated cassette Apoptosis assays EMSA: Electrophoretic mobility exchange (FRT/Flp & Cre/LoxP) Yeast two-hybrid system (gel) shift assay ChIP: Chromatin immunoprecipitation RNAi (siRNA & shRNA design) 3C: Chromosome Conformation Immunoassays Capture & ChIP-3C
Name / Title
Company / Classification
Phones & Addresses
Liang Huang Ccr Nci
U S Dept of Health and Human Services Associations
200 Independence Ave SW, Washington, DC 20201 900 2 St NE #211, Washington, DC 20201 2026190257, 2024089520
Liang Dong Huang President, Director, Principal
OLD AMIGO, INC Management Consulting Services · Nonclassifiable Establishments