Liem Thi Nguyen

US Patent:

7135289, Nov 14, 2006

Filed:

Oct 7, 2002

Appl. No.:

10/266906

Inventors:

Paul D. Taylor - Gilroy CA, US
Liem T. Nguyen - San Jose CA, US

Assignee:

Transgenomic, Inc. - Omaha NE

International Classification:

C12Q 1/68
A61K 7/075
A01N 57/26

US Classification:

435 6, 510123, 514 77

Abstract:

Methods, compositions, and kits for separating heteroduplex and homoduplex DNA molecules in a test mixture by temperature-compression denaturing high performance liquid chromatography (tcDHPLC). The method includes use of nitrogen-containing additives in the mobile phase that allow detection of diverse heteroduplex molecules to be performed at the same pre-selected temperature. An example of a preferred additive is betaine. Standard mixtures of DNA fragments, such as mutation standards containing known heteroduplex and homoduplex molecules, can be used to select the concentration of additive and temperature. Compositions and kits including the mobile phase, mutation standards, PCR primers, separation media, and DNA polymerase are also provided.

US Patent:

7623474, Nov 24, 2009

Filed:

Feb 14, 2006

Appl. No.:

11/353544

Inventors:

Thuan Van Tran - Cary NC, US
Yi Yang - Morrisville NC, US
Liem Nguyen - San Jose CA, US
Russell White - Holly Springs NC, US
Donnie Van Savage - Raleigh NC, US

Assignee:

Cisco Technology, Inc. - San Jose CA

International Classification:

H04L 12/26

US Classification:

370255, 370390

Abstract:

Techniques for sending data in a packet-switched communications network include determining multiple subsets of adjacent network nodes of the network. The adjacent network nodes communicate without intervening network nodes with a particular network node through an interface on the particular network node. Each subset includes multiple adjacent network nodes. Subset definition data is sent through the interface. The subset definition data indicates which adjacent network nodes belong to which subset. Data for fewer than all adjacent network nodes in all subsets are sent by including, in a multicast data packet sent over the interface with a multicast destination address, subset identifier data that indicates a particular subset. When such data is received by a node, it is discarded unless the subset identifier matches the receiving node's subset identifier. Among other effects, this allows routing messages to be more efficiently sent to better performing neighboring network nodes.

US Patent:

7639680, Dec 29, 2009

Filed:

Oct 4, 2001

Appl. No.:

09/971298

Inventors:

Abhay Kumar Roy - San Jose CA, US
Alexey Dimitrievich Zinin - Cupertino CA, US
Liem Hong Nguyen - Apex NC, US

Assignee:

Cisco Technology, Inc. - San Jose CA

International Classification:

H04L 12/56

US Classification:

370389, 370221, 370386, 370400, 370408, 370503, 707201

Abstract:

A method and apparatus resynchronizes a link state database (LSDB) of a non-stop forwarding (NSF) router with the LSDB of a neighboring router (“neighbor”) while maintaining an existing adjacency with the neighbor in a computer network. An out-of-band resynchronization process executes on the routers to essentially maintain the existing adjacency between the router and neighbor, rather than resetting that adjacency as defined by a conventional resynchronization approach. By keeping the adjacency “up” from the perspective of a routing protocol, such as the Open Shortest Path First routing protocol, the adjacency can be used for continued data traffic to and from the NSF router.

US Patent:

7898981, Mar 1, 2011

Filed:

Mar 31, 2006

Appl. No.:

11/394587

Inventors:

Thuan Van Tran - Cary NC, US
Liem H. Nguyen - San Jose CA, US
Donnie Van Savage - Raleigh NC, US
Yi Yang - Morrisville NC, US

Assignee:

Cisco Technology, Inc. - San Jose CA

International Classification:

H04L 12/28

US Classification:

370254, 370401, 709242

Abstract:

A system and method for enhancing the behavior of a network. In an illustrative embodiment, the network includes a distribution router and plural additional routers connected to the distribution router. The system includes a first module that is adapted to characterize the additional routers as stub routers or non-stub routers and then to provide a signal in response thereto. A second module selectively queries non-stub routers for route information and does not query stub routers for the route information. In a more specific embodiment, the second module includes a unicast module for selectively unicasting queries to the non-stub routers. A multicast module selectively multicasts queries to the non-stub routers via a modified conditional-receive algorithm.

US Patent:

20040224329, Nov 11, 2004

Filed:

Jan 8, 2004

Appl. No.:

10/754775

Inventors:

Douglas Gjerde - Saratoga CA, US
Christopher Hanna - San Francisco CA, US
Liem Nguyen - San Jose CA, US
Leon Yengoyan - San Jose CA, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200

Abstract:

The subject invention provides extraction capillaries, wherein a substantial portion of the channel is coated with a 3-dimensional solid phase extraction surface that binds an analyte. In some embodiments the extraction matrix comprises a polymer backbone with an extraction agent bound thereto. Analytes of particular relevance include biomolecules, such as proteins, polynucleotides, lipids and polysaccharides. The invention further provides devices comprising the extraction capillaries, reagents for use in conjunction with the capillaries and devices, and methods for the production and use of the capillaries and devices.

US Patent:

20050258097, Nov 24, 2005

Filed:

Jun 17, 2005

Appl. No.:

11/155464

Inventors:

Douglas Gjerde - Saratoga CA, US
Christopher Hanna - San Francisco CA, US
Jeremy Lambert - Worcester MA, US
Liem Nguyen - San Jose CA, US

International Classification:

B01D015/08

US Classification:

210635000, 210656000, 436161000, 436177000

Abstract:

The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for using the extraction columns including methods for varying parameters, such as the volume of sample loaded, the number of capture cycles performed, and the number or wash cycles performed to achieve a particular desired result.

US Patent:

20060118491, Jun 8, 2006

Filed:

Dec 1, 2005

Appl. No.:

11/292707

Inventors:

Douglas Gjerde - , US
Liem Nguyen - San Jose CA, US
Ronald Jones - Morgan Hill CA, US
Christopher Holman - Overland Park KS, US

International Classification:

B01D 15/08

US Classification:

210656000, 210198200, 436161000, 422070000, 435007100

Abstract:

The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods and devices for desalting and/or buffer exchange of a sample.

US Patent:

20080119637, May 22, 2008

Filed:

Nov 20, 2007

Appl. No.:

11/986335

Inventors:

Douglas T. Gjerde - Saratoga CA, US
Liem Nguyen - San Jose CA, US
Jeremy Lambert - Worcester MA, US

International Classification:

C07K 1/02

US Classification:

530334

Abstract:

The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a polypeptide or protein) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction medium in a column. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides resins and methods that facilitate elution of analyte in a small volume of liquid. In some embodiments, the analyte is an active protein.