Lin G Gu

US Patent:

20090111133, Apr 30, 2009

Filed:

Oct 31, 2007

Appl. No.:

11/931778

Inventors:

Lin Gu - Lake Zurich IL, US
Patrick A. Worklan - Abbott Park IL, US

Assignee:

ABBOTT LABORATORIES - Abbott Park IL

International Classification:

C12Q 1/48
G01N 31/00

US Classification:

435 15, 436 15

Abstract:

A gel filtration standard suitable for use as molecular weight markers for gel filtration chromatography for a mobile phase with denaturant. In one embodiment, the gel filtration standard comprises ovalbumin, myoglobin, and vitamin B. In another embodiment, the gel filtration standard comprises phosphorylase b, ovalbumin, and myoglobin along with a reducing agent, such as, for example, dithiothreitol to reduce the multiple peaks of phosphorylase b to a single peak. In a third embodiment, the gel filtration standard comprises phosphorylase b, ovalbumin, myoglobin, and vitamin B. The benefits of the gel filtration standards described herein include acceptable resolution values and usefulness in system suitability testing. The combination of markers can be used in day-to-day testing for system suitability in the denatured condition for GPC-HPLC analysis for many proteins.