Skills:
aseptic/sterile technique dilution calculation buffers & analytical reagents defined, selective media & plates working stock & master cell banking DNA & RNA extraction, purification, quantification UV-Vis spectrophotometry molecular cloning PCR, RT-PCR, qPCR & delta-delta-Ct analysis recombineering using galK +/- selection genotyping by PCR, sequencing & chromatogram analysis Level II Biosafety Cabinet, laminar flow bench mammalian cell culture/tissue culture & cryopreservation recombinant protein induction, overexpression, purification affinity, size exclusion, ion exchange chromatography Bradford, Lowry, BCA method bright-field, dark-field, phase contrast, fluorescence microscopy indirect & sandwich ELISA, quantitative ELISA SDS-PAGE Western Blot co-immunoprecipitation stopped-flow & spectrofluorometry Entrez, Pubmed, ATCC.org, Addgene, UCSC Genome Browser BLAST programs gel image analysis software (ImageJ) virtual cloning & sequence annotation software (DNASTARs LaserGene module: SeqBuilder) EndNote, Adobe Acrobat X Internet Explorer, Google Chrome, Firefox MS Word, Excel, PowerPoint (2003, 2007) Windows XP, Vista, 7, 8, Mac OS, Linux