Linda V Ballard

US Patent:

20040023238, Feb 5, 2004

Filed:

Nov 20, 2002

Appl. No.:

10/296012

Inventors:

Charles Keller - Salt Lake City UT, US
Linda Ballard - Salt Lake City UT, US
Richard Lemmons - Salt Lake City UT, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

A high-throughput assay for characterizing a subject's genetic makeup is disclosed. Specifically a high-throughput assay utilizing PCR is disclosed that permits the rapid and accurate characterization of a subject's inherited alleles of the polymorphic glutathione S-transferase (GST) genes GSTM1, GSTM3, GSTP1, and GSTT1. This method allows detection of the specific alleles inherited, including the gene dosage of GSTM1 and GSTT1 while not requiring restriction endonuclease digestion of the PCR products in order to detect length differences. Further, the method allows all analyses to be performed simultaneously in the same gel lane, thus further adding efficiency and cost-effectiveness.

US Patent:

20060194200, Aug 31, 2006

Filed:

Oct 15, 2003

Appl. No.:

10/530512

Inventors:

Charles Keller - SALT LAKE CITY UT, US
Linda Ballard - Salt Lake City UT, US
Richard Lemmons - Salt Lake City UT, US
Francis Ali-Osman - Houston TX, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

A high-throughput assay for characterizing a subject's genetic makeup is disclosed. Specifically, a high-throughput assay utilizing PCR is disclosed that permits the rapid and accurate characterization of a subject's inherited alleles of the polymorphic glutathione S-transferase (GST) genes GSTM, GSTM, GSTP, and GSTT. This method allows detection of the specific alleles inherited, including the gene dosage of GSTMand GSTTwhile not requiring restriction endonuclease digestion of the PCR products in order to detect length differences. Further, the method allows all analyses to be performed simultaneously in the same gel lane, thus further adding efficiency and cost-effectiveness.