Linda B Ludwig

US Patent:

6392029, May 21, 2002

Filed:

Feb 12, 1999

Appl. No.:

09/249542

Inventors:

Linda B. Ludwig - East Aurora NY
Kristie Anne Krawczyk - Gowanda NY

Assignee:

The Research Foundation of State University of New York - Amherst NY

International Classification:

C07H 2100

US Classification:

536 2372, 4241391, 4241871, 4241851, 435 6, 435 691, 4353201, 435 71, 435235, 514 44, 530300, 530350, 536 237, 536 245

Abstract:

Disclosed is a gene comprising an open reading frame encoded on the plus strand of the pro-viral DNA, and located in the region of HIV-1 long terminal repeat. The gene encodes a protein that is related to, and has a structural motif resembling that of chemokine proteins. Depending upon the ribosomal frameshift, a plurality of proteins may be translated from the antisense RNA. The protein has similarity with chemokine SDF-1 and may play a role as a cofactor with gp120 in the binding to and entry of HIV to a target cell.

US Patent:

7700725, Apr 20, 2010

Filed:

Dec 23, 2004

Appl. No.:

11/020772

Inventors:

Linda B. Ludwig - East Aurora NY, US

International Classification:

C07K 14/155
C07K 14/16
C07K 16/08
C07K 16/10

US Classification:

530350, 5303871, 5303883, 53038835

Abstract:

Disclosed is a novel HIV gene comprising a set of open reading frames encoded with the template as the plus strand of the proviral DNA, and located in the region of HIV-1 long terminal repeat. The genes encode a set of antisense proteins, (HAPs) as well as smaller proteins, related to, and containing structural motif resembling that of chemokine proteins. Depending upon the ribosomal frameshift, a plurality of proteins may be translated from the antisense RNA. The smaller proteins have similarity with chemokine SDF-1 and may play a role as a cofactor with gp120 in the binding to and entry of HIV to a target cell.

US Patent:

7700726, Apr 20, 2010

Filed:

Mar 10, 2005

Appl. No.:

11/076728

Inventors:

Linda B. Ludwig - East Aurora NY, US

International Classification:

C07K 14/155
C07K 14/16
C07K 16/08
C07K 16/10

US Classification:

530350, 5303871, 5303883, 53038835

Abstract:

Disclosed is a novel HIV gene comprising a set of open reading frames encoded with the template as the plus strand of the proviral DNA, and located in the region of HIV-1 long terminal repeat. The genes encode a set of antisense proteins, (HAPs) as well as smaller proteins, related to, and containing structural motif resembling that of chemokine proteins. Depending upon the ribosomal frameshift, a plurality of proteins may be translated from the antisense RNA. The smaller proteins have similarity with chemokine SDF-1 and may play a role as a cofactor with gp120 in the binding to and entry of HIV to a target cell.

US Patent:

20030166856, Sep 4, 2003

Filed:

Apr 30, 2002

Appl. No.:

10/135545

Inventors:

Linda Ludwig - East Aurora NY, US

International Classification:

C12Q001/70
C07H021/04
C12N007/00
C07K014/16

US Classification:

530/350000, 536/023720, 435/005000, 435/235100, 435/325000, 435/069300, 435/320100

Abstract:

Disclosed is a novel HIV gene comprising a set of open reading frames encoded with the template as the plus strand of the proviral DNA, and located in the region of HIV-1 long terminal repeat. The genes encode a set of antisense proteins, (HAPs) as well as smaller proteins, related to, and containing structural motif resembling that of chemokine proteins. Depending upon the ribosomal frameshift, a plurality of proteins may be translated from the antisense RNA. The smaller proteins have similarity with chemokine SDF-1 and may play a role as a cofactor with gp120 in the binding to and entry of HIV to a target cell.

US Patent:

59196777, Jul 6, 1999

Filed:

May 9, 1997

Appl. No.:

8/853703

Inventors:

Linda B. Ludwig - East Aurora NY

Assignee:

The Research Foundation of State University of New York - Amherst NY

International Classification:

C07H 2104
C12N 516
C12N 1511
C12P 1934

US Classification:

4351723

Abstract:

Identified are genetic regulatory elements which are part of a natural antisense RNA negative regulatory system in eukaryotic cells. The genetic regulatory elements, designated an antisense initiator sequence, when downstream of and operably linked to a DNA molecule can transcribe the DNA molecule into RNA transcripts of negative strand polarity that function to bind to, in forming an RNA duplex with, sense RNA transcripts being produced from a target gene to be regulated. The invention relates to recombinant vectors useful for introduction into eukaryotic cells, and methods of using the vectors to regulate expression of a target gene comprising introducing the recombinant vectors into host eukaryotic cells.

US Patent:

59003588, May 4, 1999

Filed:

Jul 24, 1997

Appl. No.:

8/899868

Inventors:

Linda Besante Ludwig - East Aurora NY
Barbara J. Hughes - East Aurora NY

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

A method for a non-radioactive electrophoretic mobility shift assay performed with non-radioactive labeled dsDNA, non-radioactive labeled ssDNA or non-radioactive labeled RNA probes interacting with a nucleic acid binding protein in forming a complex, electrophoresing the mixture containing the complex, transferring the complex to a membrane, and detecting the complex transferred to the membrane by detecting the non-radioactive label in the complex.

US Patent:

54847028, Jan 16, 1996

Filed:

Jan 27, 1994

Appl. No.:

8/189155

Inventors:

Linda B. Ludwig - East Aurora NY

Assignee:

Research Foundation of the State University of New York at Buffalo - Amherst NY

International Classification:

C07H 2104
C12P 1934
C12Q 148
C12Q 170

US Classification:

435 6

Abstract:

A method for preselecting a nucleic acid sequence of interest is provided. The method comprises immobilizing an affinity molecule to a solid support matrix, wherein the affinity molecule is specific for a hapten or target molecule; hybridization of a "hook" comprised of either an oligonucleotide (complementary to the sequence of interest) labeled with a hapten or target molecule to the target recombinant vector containing the nucleic acid sequence of interest; capturing of the vector-hook via the hapten or target molecule by the respective immobilized affinity molecule for which it has binding specificity; liberating the captured vector DNA by enzymatic digestion of either the hook, or of the immobilized affinity molecule; and adding and incubating competent host cells to promote the introduction of recombinant vector DNA into the competent host cells. Also disclosed are embodiments in which a) the hook is incorporated as part of the vector by hybridization to a denatured vector, followed by second strand synthesis to include the hook as part of the newly synthesized strand; and b) the hook is a protein that binds a genetic element within or adjacent to the nucleic acid sequence of interest, and the immobilized affinity molecule has binding specificity for the protein.