Liping Yu

US Patent:

8066515, Nov 29, 2011

Filed:

Nov 17, 2004

Appl. No.:

10/991169

Inventors:

Philip B. Johnson - Campbell CA, US
Liping Yu - Mountain View CA, US
Ludger Mimberg - San Jose CA, US
Ross F. Jatou - San Jose CA, US

Assignee:

NVIDIA Corporation - Santa Clara CA

International Classification:

H01R 12/00

US Classification:

439 61

Abstract:

A system provides a digital multi-bit connection between two or more graphics adapters. Each graphics adapter is manufactured as a printed circuit board including a finger-type edge connector. When two or more graphics adapters are installed in a system the edge connectors of each graphics adapter may be coupled to each other via a connection device that provides a portion of the digital multi-bit connection. The remainder of the digital multi-bit connection is provided by conductive traces coupling each finger of the edge connector to a graphics processing unit that is affixed to the graphics adapter. The connection device may be installed by an end-user as each additional graphics adapter is installed in the system.

US Patent:

20090325192, Dec 31, 2009

Filed:

Dec 15, 2008

Appl. No.:

12/316707

Inventors:

Hrair Kirakossian - San Jose CA, US
Liping Yu - San Jose CA, US
Douglas A. Petry - San Ramon CA, US

International Classification:

G01N 33/53
G01N 21/00
G01N 33/553
G01N 33/543

US Classification:

435 72, 422 8205, 436164, 436526, 436518

Abstract:

The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4T cells in a whole blood sample.

US Patent:

20130330739, Dec 12, 2013

Filed:

Apr 5, 2012

Appl. No.:

13/984019

Inventors:

Liping Yu - San Jose CA, US

Assignee:

BECTON, DICKINSON AND COMPANY - Franklin Lakes NJ

International Classification:

G01N 1/34

US Classification:

435 71, 422527, 4352871, 436501

Abstract:

Devices for separating magnetically labeled moieties in a sample are provided. Aspects of the devices include a magnetic field source, a first magnetic field guide having a wedge-shaped portion with an apex edge, and a second magnetic field guide having a wedge-shaped portion with an apex edge. The apex edge of the first magnetic field guide is aligned substantially across from and parallel to the apex edge of the second magnetic field guide, and the device is configured to separate magnetically labeled moieties from non-magnetically labeled moieties in the sample. Also provided are methods of using the devices, as well as systems and kits configured for use with the devices and methods.

US Patent:

20210395817, Dec 23, 2021

Filed:

Jul 14, 2021

Appl. No.:

17/375377

Inventors:

- Franklin Lakes NJ, US
Liping Yu - San Jose CA, US

International Classification:

C12Q 1/6874
C12Q 1/6806
G01N 15/14
C12Q 1/6804
C12Q 1/6886

Abstract:

Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.

US Patent:

20200308642, Oct 1, 2020

Filed:

Apr 30, 2020

Appl. No.:

16/863280

Inventors:

- Franklin Lakes NJ, US
Liping Yu - San Jose CA, US

International Classification:

C12Q 1/6874
C12Q 1/6806
G01N 15/14
C12Q 1/6804
C12Q 1/6886

Abstract:

Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.

US Patent:

20180136098, May 17, 2018

Filed:

Dec 26, 2017

Appl. No.:

15/854455

Inventors:

- Franklin Lakes NJ, US
Liping Yu - San Jose CA, US

International Classification:

G01N 1/34
B03C 1/035
B03C 1/033

Abstract:

Devices for separating magnetically labeled moieties in a sample are provided. Aspects of the devices include a magnetic field source, a first magnetic field guide having a wedge-shaped portion with an apex edge, and a second magnetic field guide having a wedge-shaped portion with an apex edge. The apex edge of the first magnetic field guide is aligned substantially across from and parallel to the apex edge of the second magnetic field guide, and the device is configured to separate magnetically labeled moieties from non-magnetically labeled moieties in the sample. Also provided are methods of using the devices, as well as systems and kits configured for use with the devices and methods.

US Patent:

20170363522, Dec 21, 2017

Filed:

Jun 1, 2017

Appl. No.:

15/611651

Inventors:

- Franklin Lakes NJ, US
Liping Yu - San Jose CA, US

International Classification:

G01N 1/40
B01L 3/00

Abstract:

Aspects of the present disclosure include an acoustic separator for acoustically separating components of a sample, such as a biological sample. Acoustic separators according to certain embodiments include an acoustic field generator soldered to a flow channel having a fluid flow path where the acoustic field generator produces an acoustic field in the fluid flow path. Methods of soldering an acoustic field generator to a flow channel having a fluid flow path are also described. Methods for acoustically separating components of a sample as well as systems and kits, including one or more acoustic separators, suitable for practicing the subject methods are also provided.

US Patent:

20150177111, Jun 25, 2015

Filed:

Dec 11, 2014

Appl. No.:

14/567892

Inventors:

- Franklin Lakes NJ, US
Liping Yu - San Jose CA, US
Smita Ghanekar - Fremont CA, US
Jianying Cao - San Jose CA, US

International Classification:

G01N 1/40
G01N 15/14
G01N 1/28

Abstract:

Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include disrupting a biological sample to produce a disrupted biological sample and acoustically separating larger components from smaller components in the disrupted biological sample. In certain embodiments, methods may include monitoring aggregation while the biological sample is being processed. Methods, in certain instances, also include acoustically separating cells from cellular debris and non-cellular macromolecules as well as magnetically separating magnetically labelled moieties from unlabeled moieties. Systems, including a disrupter, one or more acoustic concentrator devices, feedback monitors and magnetic separation devices suitable for practicing the subject methods are also described.