Lisa Diane Mcdaniel

US Patent:

20110086772, Apr 14, 2011

Filed:

Sep 27, 2010

Appl. No.:

12/891623

Inventors:

Lisa McDaniel - Spokane WA, US
Blake Ballif - Greenacres WA, US
Roger Schultz - Spokane WA, US
Brice Tebbs - Spokane WA, US
Bassem Bejjani - Spokane WA, US
Lisa Shaffer - Colbert WA, US

Assignee:

Signature Genomics Laboratories LLC - Spokane WA

International Classification:

C40B 30/04
C40B 40/08

US Classification:

506 9, 506 17

Abstract:

Multiplex (+/−) stranded analyses, such as array comparative genomic hybridization (aCGH), are provided for detecting chromosomal rearrangements associated with cancer and other diseases. For example, an illustrative multiplex array for CGH includes discrete plus (+) strand and minus (−) strand DNA probes, complementary to each other but separable on the CGH array. The minus (−) strand DNA probes recover diagnostic information lost to conventional microarrays, since many genes transcribe from the minus (−) strand. In an illustrative system, patient and control DNA samples are prepared for CGH by amplification and labeling using comprehensive primers that generate both plus (+) strands and minus (−) strands of DNA in the samples. The breakpoints of a translocated chromosome may be detected on a multiplex microarray by DNA probes of one polarity, while DNA copy number changes associated with the translocation region may be detected by corresponding DNA probes of the complementary polarity. Related methods for identifying translocation partner genes are also provided.