Louis Anthony Clark

US Patent:

8216815, Jul 10, 2012

Filed:

Jun 29, 2010

Appl. No.:

12/825939

Inventors:

Robert McDaniel - Palo Alto CA, US
Behnaz Behrouzian - Sunnyvale CA, US
Louis Clark - San Francisco CA, US
Douglas Hattendorf - Mountain View CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12P 7/42
C12P 21/06
C12P 21/04
C12Q 1/68
C12Q 1/26
C12N 9/02
C12N 1/20
C12N 15/00
C07H 21/04

US Classification:

435146, 435 61, 435189, 4352523, 4353201, 435440, 435 691, 435 711, 435 25, 536 232

Abstract:

The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as and ; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.

US Patent:

8298795, Oct 30, 2012

Filed:

Aug 22, 2011

Appl. No.:

13/215193

Inventors:

Jie Yang - Redwood City CA, US
Louis Clark - San Francisco CA, US
Onorato Campopiano - Hayward CA, US
Kripa Rao - San Mateo CA, US
Lorand Szabo - Budapest, HU
Janos Torok - Gödöllö, HU
Dipnath Baidyaroy - Fremont CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12P 19/14
C12P 1/00
C12P 7/14
C12N 9/42

US Classification:

435 99, 435 41, 435162, 435163, 435209, 530350

Abstract:

The invention provides recombinant GH61 proteins obtained from , and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

US Patent:

8357523, Jan 22, 2013

Filed:

Jun 16, 2010

Appl. No.:

12/816989

Inventors:

Sally Rhiannon Postlethwaite - Redwood City CA, US
Louis Clark - San Francisco CA, US
Catherine M. Cho - Redwood City CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/26

US Classification:

435201

Abstract:

The invention provides variants of the CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.

US Patent:

8530201, Sep 10, 2013

Filed:

Dec 19, 2012

Appl. No.:

13/719431

Inventors:

Louis Clark - San Francisco CA, US
Catherine M. Cho - Redwood City CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12P 19/14
C12P 19/22
C12P 19/20
C12P 19/18
C12P 19/16
C12P 19/02

US Classification:

435 99, 435 95, 435 96, 435 97, 435 98, 435105

Abstract:

The invention provides variants of the CelA β-glucosidase that have improve β-glucosidase activity, particularly improved thermoactivity, compared to the wild type enzyme. The invention further provides related polynucleotides, vectors, host cell, and methods for making and using the variants.

US Patent:

8574877, Nov 5, 2013

Filed:

Jun 12, 2012

Appl. No.:

13/494709

Inventors:

Robert McDaniel - Palo Alto CA, US
Behnaz Behrouzian - Sunnyvale CA, US
Louis Clark - San Francisco CA, US
Douglas A. Hattendorf - Mountain View CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12P 7/42
C12P 21/06
C12P 21/04
C12Q 1/68
C12Q 1/26
C12N 9/02
C12N 1/20
C12N 15/00
C07H 21/04

US Classification:

435146, 435 61, 435189, 4352523, 4353201, 435440, 435 691, 435 711, 435 25, 536 232

Abstract:

The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as and ; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.

US Patent:

8574878, Nov 5, 2013

Filed:

Jun 28, 2011

Appl. No.:

13/171138

Inventors:

Behnaz Behrouzian - Sunnyvale CA, US
Louis Clark - San Francisco CA, US
Yihui Zhu - Redwood City CA, US
Michael Clay - Menlo Park CA, US
Kristian Karlshoej - Santa Clara CA, US

International Classification:

C12P 7/04
C12P 5/02
C12P 7/42
C12P 21/06
C12N 9/02
C12N 1/20
C12N 1/00
C11D 3/00
C07C 51/00
C07C 53/00
C07C 1/00
C07C 29/00

US Classification:

435157, 435167, 435146, 435189, 435 691, 43525233, 4352523, 43525411, 510392, 510505, 554124, 554 1, 568840, 585733, 585639

Abstract:

The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.

US Patent:

8580555, Nov 12, 2013

Filed:

Apr 20, 2012

Appl. No.:

13/452328

Inventors:

Erik De Vries - Livermore CA, US
Louis Clark - San Francisco CA, US
Scott McVicar - Sunnyvale CA, US
Erika Segraves - Sunnyvale CA, US
Shiwei Song - Singapore, SG
Kheng Lin Tan - Singapore, SG

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/88
C12P 7/02
C12P 7/22
C12P 7/04
C12P 21/06
C07H 21/04
C07K 1/00

US Classification:

435232, 435155, 435156, 435157, 435 691, 536 232, 530350

Abstract:

The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.

US Patent:

20100173372, Jul 8, 2010

Filed:

Dec 18, 2009

Appl. No.:

12/642586

Inventors:

Erik De Vries - Livermore CA, US
Louis Clark - San Francisco CA, US
Scott McVicar - Sunnyvale CA, US
Erika Segraves - Sunnyvale CA, US
Shiwei Song - Singapore, SG
Kheng Lin Tan - Singapore, SG

Assignee:

CODEXIS, INC. - Redwood City CA

International Classification:

C12P 7/02
C12N 9/88
C07H 21/04
C12N 15/63
C12N 5/10

US Classification:

435155, 435232, 536 232, 4353201, 435325

Abstract:

The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.