Lyle L Brown

US Patent:

55896235, Dec 31, 1996

Filed:

Dec 20, 1994

Appl. No.:

8/360974

Inventors:

Adolph J. Ferro - Lake Oswego OR
Richard K. Bestwick - Portland OR
Lyle R. Brown - Corvallis OR

Assignee:

Agritope, Inc. - Beaverton OR

International Classification:

A01H 400
C12N 1582

US Classification:

800205

Abstract:

A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and, transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence of the AdoMetase gene and the expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability. The current construction of transgenic plants containing a copy(s) of the T3 AdoMetase gene allow for construction of plants that will control ethylene biosynthesis under restricted conditions resulting in fruits, vegetables, and flowers which have been modified internally to improve shelf life and preservation qualities.

US Patent:

54162505, May 16, 1995

Filed:

Jun 8, 1994

Appl. No.:

8/255833

Inventors:

Adolph J. Ferro - Lake Oswego OR
Richard K. Bestwick - Portland OR
Lyle R. Brown - Corvallis OR

Assignee:

Agritope, Inc. - Beaverton OR

International Classification:

A01H 400
C12N 1582

US Classification:

800205

Abstract:

A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and, transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence of the AdoMetase gene and the expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability. The current construction of transgenic plants containing a copy(s) of the T3 AdoMetase gene allow for construction of plants that will control ethylene biosynthesis under restricted conditions resulting in fruits, vegetables, and flowers which have been modified internally to improve shelf life and preservation qualities.