David Miles - San Diego CA, US Lyle Turner - Cardiff CA, US Robert Marcil - Carlsbad CA, US Gina McConnell - San Diego CA, US
Assignee:
Invitrogen Corporation
International Classification:
C12N015/85 C12P019/34
US Classification:
435/091200, 435/320100, 435/455000
Abstract:
The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.
System For The Rapid Manipulation Of Nucleic Acid Sequenaces
David Miles - San Diego CA, US Lyle Turner - Cardiff CA, US Robert Marcil - Carlsbad CA, US Gina McConnell - San Diego CA, US
Assignee:
Invitrogen Corporation - Carlsbad CA
International Classification:
C12Q001/68 C12P019/34
US Classification:
435006000, 435091100
Abstract:
The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.
System For The Rapid Manipulation Of Nuculeic Acid Sequences
David Miles - San Diego CA, US Lyle Turner - Cardiff CA, US Robert Marcil - Carlsbad CA, US Gina McConnell - San Diego CA, US
Assignee:
Invitrogen Corporation - Carlsbad CA
International Classification:
C12N 15/09
US Classification:
435455000
Abstract:
The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.
System For The Rapid Manipulation Of Nucleic Acid Sequences
David Miles - San Diego CA, US Lyle Turner - Cardiff CA, US Robert Marcil - Carlsbad CA, US Gina McConnell - San Diego CA, US
Assignee:
INVITROGEN CORPORATION
International Classification:
C12N015/863
US Classification:
435/455000, 435/456000, 435/320100
Abstract:
The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.
WisdomTools, Inc. since Jul 2000
Dir. of Development Operations
Education:
Indiana University Bloomington 1997 - 1999
Indiana University Bloomington 1989 - 1994
Skills:
Information Architecture Multimedia Interaction Design Project Management User Experience User Interface Design Usability E Learning Usability Testing Instructional Design Web Design User Experience Design User Centered Design Human Computer Interaction Product Management Product Support Editing Content Management Needs Analysis Photography Html Storyboarding Wireframes
Interests:
Questions That Contain Assumptions Why Did X Fail Openid Identity The Internet Macheist
eadle has long been a supporter of Democratic causes, donating hundreds of thousands of dollars to Democratic candidates at all levels of government. She and her ex-husband, Lyle Turner, were reportedly among the early investors in a venture that eventually became Gores cable channel CurrentTV.