Lynn Clarice Thomason from 603 Lee Pl, Frederick, MD 21702, age 71, Phone: 3016684863

Dna Recombination In Eukaryotic Cells By The Bacteriophage Phic31 Recombination System
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US Patent:

8129598, Mar 6, 2012
Filed:

Nov 24, 2003
Appl. No.:

10/721980
Inventors:

David W. Ow - Hercules CA, US
Richard Calendar - Berkeley CA, US
Lynn Thomason - Albany CA, US
Assignee:

The Regents of the University of California - Oakland CA
The United States of America as represented by the Secretary of Agriculture - Washington DC
International Classification:

C12N 5/04
US Classification:

8003173, 4352542, 435462, 435468, 435471, 435419, 4352351, 435475, 435410
Abstract:

This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site-specific recombination systems that use prokaryotic recombinase polypeptides, such as the ΦC31 integrase, that can mediate recombination between the recombination sites, but not between hybrid recombination sites that are formed upon the recombination. Thus, the recombination is irreversible in the absence of additional factors. Eukaryotic cells that contain the recombinase polypeptides, or genes that encode the recombinases, are also provided.

Dna Recombination In Eukaryotic Cells By The Bacteriophage Phic31 Recombination System
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US Patent:

6746870, Jun 8, 2004
Filed:

Jul 21, 2000
Appl. No.:

09/620800
Inventors:

David W. Ow - Hercules CA
Richard Calendar - Berkeley CA
Lynn Thomason - Albany CA
Assignee:

The Regents of the University of California - Oakland CA
The United States of America as represented by the Secretary of Agriculture - Washington DC
International Classification:

C12N 1574
US Classification:

435477, 435471, 4353201, 43525235
Abstract:

This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site-specific recombination systems that use prokaryotic recombinase polypeptides, such as the C31 integrase, that can mediate recombination between the recombination sites, but not between hybrid recombination sites that are formed upon the recombination. Thus, the recombination is irreversible in the absence of additional factors. Eukaryotic cells that contain the recombinase polypeptides, or genes that encode the recombinases, are also provided.