Malcolm W Martin

US Patent:

47525657, Jun 21, 1988

Filed:

Apr 7, 1986

Appl. No.:

6/849059

Inventors:

Thomas M. Folks - Gaithersburg MD
Douglas M. Powell - Silver Spring MD
Malcolm A. Martin - Bethesda MD

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C12Q 170
C12N 1500
C12N 704
C12N 500

US Classification:

435 5

Abstract:

Leu-3. sup. - cells surviving infection with the AIDS retrovirus can be induced with IUdR to express infectious virus. A cellular clone (8E5), isolated by limiting dilution of a mass culture of survivor cells, was found to contain a single, integrated, defective provirus that was consitutively expressed. Although IUdR treatment of 8E5 cells failed to induce infectious virus, cocultivation with Leu-3. sup. + generated the characteristic syncytia associated with acute AIDS retrovirus infention. The single integrated copy of proviral DNA directs the synthesis of all major viral structural proteins except p64 and p34 as monitored by immunoblotting. Diagnostic reagents and kits in accordance with the present invention are also described.

US Patent:

49313932, Jun 5, 1990

Filed:

Sep 14, 1987

Appl. No.:

7/095837

Inventors:

Malcolm A. Martin - Bethesda MD
Ronald Willey - Alexandria VA

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C12N 700
C12N 1500
C07H 1512

US Classification:

435235

Abstract:

The present invention is related to providing a non-infectious molecular clone of a mutant HIV and HIV proteins useful as immunogens and reagents for diagnostic kit.