Man H Cheng

US Patent:

20110104760, May 5, 2011

Filed:

Jan 7, 2010

Appl. No.:

12/683950

Inventors:

Xiao-Song Gong - Berkeley CA, US
John Sullivan - Oakland CA, US
Rongdian Fu - El Cerrito CA, US
Yan Wang - San Francisco CA, US
Evan H. Bursey - Concord CA, US
Man Cheng - San Ramon CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12P 19/34
C12N 9/96
C12N 9/12
C07K 16/00
C07H 21/04

US Classification:

435 912, 435188, 435194, 5303879, 536 2353

Abstract:

The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

US Patent:

20120258460, Oct 11, 2012

Filed:

Feb 10, 2012

Appl. No.:

13/371126

Inventors:

Man Cheng - San Ramon CA, US
Yan Wang - San Francisco CA, US
John Sullivan - Oakland CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12Q 1/68
C12N 9/96

US Classification:

435 612, 435188

Abstract:

Improved Sso7-polymerase conjugate proteins are provided

US Patent:

20120258500, Oct 11, 2012

Filed:

Feb 10, 2012

Appl. No.:

13/370604

Inventors:

Man Cheng - San Ramon CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12P 19/34
C12N 9/12

US Classification:

435 912, 435194

Abstract:

The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.

US Patent:

20130190195, Jul 25, 2013

Filed:

Jul 23, 2012

Appl. No.:

13/555898

Inventors:

Man Cheng - San Ramon CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12Q 1/68

US Classification:

506 9, 435 612, 506 16

Abstract:

Particles that generate electromagnetic spectra upon appropriate illumination and in which the spectra can be converted to codes that reflect differences in the spectra are functionalized with a plurality of identical primer pairs on which bridge amplification can be performed. Using these particles, nucleic acids in a biological sample are amplified and exposed to a label that sends a signal only when bound to double-stranded DNA. The code for each particle is correlated with the primer pairs, and the captured nucleic acids are thus detected and identified. The procedure is useful for multiplex DNA or RNA detection as well as counting of target DNA and RNA molecules in the sample.

US Patent:

20100136559, Jun 3, 2010

Filed:

Nov 13, 2009

Appl. No.:

12/618076

Inventors:

Steve Okino - San Carlos CA, US
Man Cheng - San Ramon CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present application provides methods and compositions for determining accessibility of a DNA modifying agent in genomic DNA.

US Patent:

20210403989, Dec 30, 2021

Filed:

Jun 24, 2021

Appl. No.:

17/357618

Inventors:

- Hercules CA, US
Man CHENG - Danville CA, US

International Classification:

C12Q 1/6834
C12Q 1/6869

Abstract:

Barcoding composition and methods involving solid supports having sets of different oligonucleotides that can be decoded to the same identification sequence.

US Patent:

20200385791, Dec 10, 2020

Filed:

Jun 8, 2020

Appl. No.:

16/896118

Inventors:

- Hercules CA, US
Zachary BURKETT - Walnut Creek CA, US
Man CHENG - Danville CA, US
Meiye WU - Tracy CA, US
Pranav PATEL - Fremont CA, US
Duc DO - San Jose CA, US
Arkadiusz BIBILLO - Walnut Creek CA, US

International Classification:

C12Q 1/6834
C12Q 1/6874
C12Q 1/6876
C12N 15/10

Abstract:

Provided are methods for detecting the presence of multiple barcoded solid supports in partitions, the method comprising providing a plurality of partitions wherein at least some partitions comprises multiple solid supports, where each solid support is linked to different solid support oligonucleotides, the solid support oligonucleotides comprising a barcode unique for the solid support; synthesizing a complementary sequence of the oligonucleotides to produce a double-stranded polynucleotide having barcodes from two of the solid support oligonucleotides; and sequencing one or both strands of the double-stranded polynucleotides, wherein sequences comprising two different barcodes indicates the presence of two solid supports in the same partition. Also provided are compositions and methods for producing the oligonucleotides.

US Patent:

20190225951, Jul 25, 2019

Filed:

Jan 15, 2019

Appl. No.:

16/248442

Inventors:

- Hercules CA, US
Man CHENG - Danville CA, US

International Classification:

C12N 9/12
C12Q 1/6876

Abstract:

Mutant Type-A DNA polymerases having increased resistance to one or more polymerization activity inhibitors are provided.