Marcelo B Sztein

US Patent:

6733762, May 11, 2004

Filed:

May 3, 2001

Appl. No.:

09/786319

Inventors:

Alessio Fasano - West Friendship MD
Marcelo B. Sztein - Columbia MD
Ruiliang Lu - Baltimore MD
Michael K. Tanner - Crestwood KY

Assignee:

University of Maryland, Baltimore - Baltimore MD

International Classification:

A61K 39106

US Classification:

4242611

Abstract:

Methods of using Zot or zonulin as an antigen specific inhibitor of APC activity and lymphocyte proliferation, being primarily useful in the field of immunoregulation and immunotherapy as described. Specifically, Zot and zonulin inhibit antigen presenting cell-mediated antigen-specific lymphocyte proliferation in a dose dependent manner. This effect is associated with the presence of a macrophage surface receptor to which Zot binds in a specific and saturable way. This down-regulation of the immune response is, at least in part, associated with a decreased uptake of antigen.

US Patent:

20120196275, Aug 2, 2012

Filed:

Jan 27, 2012

Appl. No.:

13/360539

Inventors:

Rosangela MEZGHANNI - Ellicott City MD, US
Alessio Fasano - West Friendship MD, US
Marcelo Sztein - Baltimore MD, US

International Classification:

C12Q 1/02
C12Q 1/70
C12Q 1/68
C12N 5/071
C12Q 1/42

US Classification:

435 5, 435371, 435347, 435 29, 435 21, 435 61

Abstract:

Disclosed are methods of preparing multi-cellular three-dimensional tissue constructs, that include fibroblasts, endothelial cells, lymphocytes and epithelial cells. The present methods may include embedding fibroblasts and endothelial cells in a matrix enriched with gut basement membrane proteins to form a cell containing matrix that is then added to a bioreactor and exposed to epithelial cells and activated lymphocytes as the cell cultures. Also provided are the tissue constructs formed from such methods, a matrix enriched with gut basement membrane proteins and kits that include the same. Further provided are methods of measuring toxicity of a pathogen or commensal organisms, chemosensitivity of tissues to a toxic material and inflammatory conditions, which use the present multi-cellular three-dimensional tissue constructs.

US Patent:

61906697, Feb 20, 2001

Filed:

May 13, 1998

Appl. No.:

9/076761

Inventors:

Fernando R. Noriega - Baltimore MD
Marcelo B. Sztein - Columbia MD
Myron M. Levine - Columbia MD

Assignee:

University of Maryland, Baltimore - Baltimore MD

International Classification:

A61K 39112

US Classification:

4242581

Abstract:

Attenuated Salmonella mutants which constitutively express the Vi antigen are disclosed, as well as vaccines against typhoid fever containing the same, live vector vaccines containing the same, and DNA-mediated vaccines containing the same.