Margaret L Kirby

US Patent:

20020114784, Aug 22, 2002

Filed:

Jan 4, 2002

Appl. No.:

10/038984

Inventors:

Yin-Xiong Li - Durham NC, US
Michael Farrell - Canoga Park CA, US
Margaret Kirby - Durham NC, US

Assignee:

Medical College of Georgia Research Institute, Inc. - Augusta GA

International Classification:

A61K048/00
C12N015/86

US Classification:

424/093200, 435/456000, 435/455000

Abstract:

Introduction of double stranded RNA into cells, cell culture, organs and tissues, and whole organisms, particularly vertebrates, specifically attenuates gene expression.

US Patent:

20050118602, Jun 2, 2005

Filed:

Oct 8, 2003

Appl. No.:

10/681479

Inventors:

Yin-Xiong Li - Durham NC, US
Margaret Kirby - Durham NC, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435006000, 435091200

Abstract:

An mRNA is amplified by (a) binding a first primer to a target mRNA, the first primer comprising, in the 5′ to 3′ direction, a first known segment and an oligo T segment; (b) transcribing a cDNA from said target mRNA by elongation of said first primer with reverse transcriptase; and then (c) linking a second known segment to the 3′ terminus of said cDNA. In a preferred embodiment the step of transcribing a cDNA from said target mRNA is carried out so that at least one additional C residue is produced on the 3′ terminus of said cDNA, and the said step of linking a second known segment to the 3′ terminus of said cDNA is carried out by: (i) binding a second bridge primer to said cDNA, said second primer comprising, in the 5′ to 3′ direction, a second known segment and at least one G residue, said second primer having an inactivated G residue on the 3′ terminus thereof; and then (ii) further transcribing said cDNA from second bridge primer by elongation of said at least one additional C residue with reverse transcriptase so that a cDNA is produced having said first known segment on the 5′ terminus thereof and said second known segment on the 3′ terminus thereof. The method can be used to amplify a plurality of mRNAs together, even though a small sample of mRNAs is available such as obtained from a single cell or multiple cells obtained by laser capture microdissection from tissue or organs, and the product of the amplification then used for gene family analysis or microarray expression analysis.

US Patent:

20130251693, Sep 26, 2013

Filed:

May 22, 2012

Appl. No.:

13/477429

Inventors:

YIN-XIONG LI - Durham NC, US
Michael J. Farrell - Canoga Park CA, US
Margaret L. Kirby - Durham NC, US

Assignee:

Georiga Health Sciences Univ. Research Inst., Inc. - Augusta GA

International Classification:

C12N 15/113
A61K 48/00

US Classification:

424 9321, 435375, 435349, 435354, 435353

Abstract:

Introduction of double stranded RNA into cells, cell culture, organs and tissues, and whole organisms, particularly vertebrates, specifically attenuates gene expression.

US Patent:

20150152413, Jun 4, 2015

Filed:

Sep 5, 2014

Appl. No.:

14/478338

Inventors:

YIN-XIONG LI - DURHAM NC, US
MICHAEL J. FARRELL - CANOGA PARK CA, US
MARGARET L. KIRBY - DURHAM NC, US

International Classification:

C12N 15/113

Abstract:

Introduction of double stranded RNA into cells, cell culture, organs and tissues, and whole organisms, particularly vertebrates, specifically attenuates gene expression.