Margaret L Mcintosh

US Patent:

54609410, Oct 24, 1995

Filed:

Jul 12, 1993

Appl. No.:

8/089910

Inventors:

Rafael D. Camerini-Otero - Kensington MD
Margaret McIntosh - Chevy Chase MD
Carol S. Camerini-Otero - Kensington MD
Lance J. Ferrin - Rockville MD

Assignee:

The United States of America, as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12N 1500
C12N 1509

US Classification:

435 6

Abstract:

The present invention relates to a method of forming a three-stranded DNA molecule wherein each strand of the three-stranded DNA molecule is hybridized (that is, non-covalently bound) to at least one other strand of the three-stranded DNA molecule. The method comprises: contacting a recombination protein with a double-stranded DNA molecule and with a single-stranded DNA molecule sufficiently complementary to one strand of the double-stranded DNA molecule to hybridize therewith, which contacting is effected under conditions such that the single-stranded DNA molecule hybridizes to the double-stranded molecule so that the three stranded DNA molecule is formed.