Marilyn L Bach

US Patent:

41247019, Nov 7, 1978

Filed:

Jul 11, 1977

Appl. No.:

5/814316

Inventors:

Marilyn L. Bach - Madison WI
Fritz H. Bach - Madison WI

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

G01N 3100
G01N 3316
C12K 900

US Classification:

424 12

Abstract:

A method for preparing a reagent for primed LD typing which comprises sensitizing purified human blood leukocytes to a secondary proliferative response by incubating them, in vitro, in mixed leukocyte culture, with lymphoblastoid cell lines from homozygous typing cells, or from other cells of specified genetic type homozygous for HLA-D, or other continuous line.

US Patent:

42658730, May 5, 1981

Filed:

Jul 3, 1978

Appl. No.:

5/921487

Inventors:

Michael J. Sheehy - Madison WI
Paul M. Sondel - Boston MA
Fritz H. Bach - Madison WI
Marilyn L. Bach - Madison WI
Rudolf Wank - New York NY

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

G01N 3316
A61K 4300

US Classification:

424 1

Abstract:

A cellular method for typing lymphocyte defined human leukocyte antigens by utilizing human blood leukocytes which have been sensitized to proliferatively respond to the secondary stimulus of the leukocyte antigens to which they have been sensitized. The sensitization is accomplished by incubating, in vitro, in a mixed leukocyte culture, the leukocytes to be sensitized with leukocytes differing by only a single major histocompatibility complex haplotype or a single HLA-D antigenic determinant.