Skills:
TECHNICAL SKILLS : Tissue Culture/Animal Isolation and culture of primary and other cell lines Growth of cells in 3-Dimensional cell culture system Initiation of tumor in mice, drug administration and dissection Molecular Biology DNA Isolation and Analysis, Electrophoresis, Southern blots, Cloning RNA isolation, RT-PCR, Real-time PCR-RNA interference Chromatin Immunoprecipitation (ChIP) assay, electro-mobility shift assay Radio-Isotopes like 3H, 35S and 32P use for various assay Cell Biology Over expression of proteins in bacterial, mammalian and baculovirus systems Cell based bioassays and Biochemical assays, ELISA, Shear flow assays Immunostaining: IHC-P, ICC, IF and Flow cytometry Confocal microscopy SDS-PAGE, protein interaction assays COMPUTER BASED TRAINING Trained on several research related software like photoshop, Microsoft office, NIH Image, PRISM, Flow jo, Graphpad Trained in Peoplesoft for the ordering and procurement in the university of Minnesota financial system.