Mark C Glaum

US Patent:

20120093781, Apr 19, 2012

Filed:

Mar 12, 2010

Appl. No.:

13/255599

Inventors:

Shyam S. Mohapatra - Lutz FL, US
Mark Glaum - Lutz FL, US
Guoqing Liu - Tampa FL, US

Assignee:

University of South Florida - Tampa FL

International Classification:

A61K 35/14
C12Q 1/68
C12Q 1/18
G01N 33/566
C12N 5/0787
C12Q 1/02

US Classification:

424 937, 435372, 435 29, 435 32, 435 792, 435 612

Abstract:

The subject invention pertains to the development of a novel human mast cell line, USF-MC1. USF-MC1 is a mast cell precursor present in human umbilical cord blood (HUCB) that may be sustained in culture in the absence of exogenous cytokines to serve as a convenient experimental model of human mast cell activation. The SCF-independent human mast cell line USF-MC1 responds to IgE-mediated and IgE-independent stimuli in a way comparable to that of LAD2. USF-MC1 cells are useful for investigation of IgE-mediated activation mechanisms of human mast cells, contributing to the development of effective treatments for allergic disorders and other disorders. The subject invention provides a ready source of human mast cells for research, including pharmacological studies for the screening of various agents, and toxicologic, e.g., for the cosmetic and pharmaceutical industries. The mast cells can also be used as biofactories, for the large-scale production of biomolecules.