Mark A Levy

US Patent:

6455288, Sep 24, 2002

Filed:

Apr 8, 1993

Appl. No.:

08/044857

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Dade Behring Marburg GmbH - Marburg
Genencor International, Inc. - San Francisco CA

International Classification:

C12N 1552

US Classification:

435190, 4352523, 4353201, 536 232

Abstract:

The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

US Patent:

7037824, May 2, 2006

Filed:

May 13, 2004

Appl. No.:

10/844533

Inventors:

Lloyd G. Burrell - Poughkeepsie NY, US
Jeffrey P. Gambino - Westford VT, US
Hyun Koo Lee - LaGrangeville NY, US
Mark D. Levy - Essex Junction VT, US
Baozhen Li - South Burlington VT, US
Stephen E. Luce - Underhill VT, US
Thomas L. McDevitt - Underhill VT, US
Anthony K. Stamper - Williston VT, US
Kwong Hon Wong - Wappingers Falls NY, US
Sally J. Yankee - Underhill VT, US

Assignee:

International Business Machines Corporation - Armonk NY

International Classification:

H01L 21/4763

US Classification:

438627, 438629, 438643, 438648, 438653, 438687

Abstract:

Tungsten studs of a size comparable to vias are provided to integrate and interface between copper and aluminum metallization layers in an integrated circuit and/or package therefor by lining a via opening, preferably with layers of tantalum nitride and PVD tungsten as a barrier against the corrosive effects of tungsten fluoride on copper. The reduced size of the tungsten studs relative to known interface structures allows wiring and connection pads to be formed in a single aluminum layer, improving performance and reducing process time and cost.

US Patent:

7087997, Aug 8, 2006

Filed:

Mar 12, 2001

Appl. No.:

09/805027

Inventors:

Lloyd G. Burrell - Poughkeepsie NY, US
Jeffrey P. Gambino - Westford VT, US
Hyun Koo Lee - LaGrangeville NY, US
Mark D. Levy - Essex Junction VT, US
Baozhen Li - South Burlington VT, US
Stephen E. Luce - Underhill VT, US
Thomas L. McDevitt - Underhill VT, US
Anthony K. Stamper - Williston VT, US
Kwong Hon Wong - Wappingers Falls NY, US
Sally J. Yankee - Underhill VT, US

Assignee:

International Business Machines Corporation - Armonk NY

International Classification:

H01L 23/48
H01L 23/52
H01L 29/40

US Classification:

257750, 257761, 257762, 257763, 257764, 257770

Abstract:

Tungsten studs of a size comparable to vias are provided to integrate and interface between copper and aluminum metallization layers in an integrated circuit and/or package therefor by lining a via opening, preferably with layers of tantalum nitride and PVD tungsten as a barrier against the corrosive effects of tungsten fluoride on copper. The reduced size of the tungsten studs relative to known interface structures allows wiring and connection pads to be formed in a single aluminum layer, improving performance and reducing process time and cost.

US Patent:

20050164402, Jul 28, 2005

Filed:

Jan 13, 2005

Appl. No.:

11/036707

Inventors:

Christopher Belisle - Concord CA, US
John Walker - Hayward CA, US
Sarah Ngola - Sunnyvale CA, US
Douglas Greiner - Fremont CA, US
Mark Levy - San Jose CA, US
Xiaoxia Zhao - Fremont CA, US
Irene Chen - Fremont CA, US
Mark Stolowitz - Pleasanton CA, US
Donald Paquin - San Jose CA, US

International Classification:

G01N001/00

US Classification:

436174000, 422099000

Abstract:

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.

US Patent:

20080248589, Oct 9, 2008

Filed:

May 19, 2005

Appl. No.:

11/596285

Inventors:

Christopher M. Belisle - Concord CA, US
John A. Walker - Hayward CA, US
Sarah M. Ngola - Sunnyvale CA, US
Douglas P. Greiner - Fremont CA, US
Mark J. Levy - San Jose CA, US
Xiaoxia Zhao - Fremont CA, US
Irene Y. Chen - Fremont CA, US
Mark L. Stolowitz - Pleasanton CA, US
Donald P. Paquin - San Jose CA, US

International Classification:

G01N 33/543
B01J 19/00

US Classification:

436518, 422 681

Abstract:

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.

US Patent:

20110070659, Mar 24, 2011

Filed:

May 24, 2010

Appl. No.:

12/786133

Inventors:

Christopher M. Belisle - Concord CA, US
Sarah M. Ngola - Sunnyvale CA, US
Douglas P. Greiner - Fremont CA, US
Mark J. Levy - San Jose CA, US
Xiaoxia Zhao - Fremont CA, US
Irene Y. Chen - Fremont CA, US
Mark L. Stolowitz - Pleasanton CA, US
Donald P. Paquin - San Jose CA, US

Assignee:

Qiagen Sciences, Inc. - Hilde

International Classification:

G01N 33/543
G01N 1/00
G01N 33/50
B01L 9/00
G01N 21/00
G01N 21/76
G01N 24/00
G01N 30/96

US Classification:

436501, 436174, 436 86, 422566, 436164, 436172, 436173, 422 69

Abstract:

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.

US Patent:

60338903, Mar 7, 2000

Filed:

May 22, 1995

Appl. No.:

8/445463

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Behring Diagnostics GmbH - Marburg

International Classification:

C12N 904

US Classification:

435190

Abstract:

The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD. sup. + dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

US Patent:

60905670, Jul 18, 2000

Filed:

May 22, 1995

Appl. No.:

8/445464

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Behringwerke AG - Marburg

International Classification:

G01N 3353
G01N 33542

US Classification:

435 79

Abstract:

Methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD. sup. + dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.