Mark M Manak

US Patent:

20020197631, Dec 26, 2002

Filed:

Apr 26, 2002

Appl. No.:

10/134054

Inventors:

Nathan Lawrence - Timonium MD, US
Feng Tao - German Town MD, US
Allan Kakita - Huntington Beach CA, US
Mark Manak - Laurel MD, US
Richard Schumacher - South Easton MA, US
James Laugharn - Winchester MA, US

International Classification:

C12Q001/68
B01L003/00
C12M001/00

US Classification:

435/006000, 422/099000, 422/101000, 436/177000, 435/287200

Abstract:

Devices and methods are described for homogenization, processing, detection, and analysis of biological samples such as insects, fungi, bacteria, and plant and animal tissues. Multiple chambers in these devices permit different processing functions to be carried out at each stage, such that the resulting homogenized product can be further processed, purified, analyzed, and/or biomolecules such as metabolites, proteins and nucleic acids, or pharmaceutical products can be detected. The device can be used in a hydrostatic pressure apparatus, in which different activities, i.e. incubations, addition or renewal of reagent, and generation and detection of signal can be carried out in the appropriate chamber. The method improves the preservation of biomolecules from chemical and enzymatic degradation relative to conventional means. Additionally, this method enables automated sample preparation and analytical processes.

US Patent:

20040072148, Apr 15, 2004

Filed:

Apr 7, 2003

Appl. No.:

10/407897

Inventors:

Jiuping Ji - Rockville MD, US
Mark Manak - Laurel MD, US
Irene Gonzalez - Baltimore MD, US

International Classification:

C12Q001/70
C12P019/34

US Classification:

435/005000, 435/091200

Abstract:

The present invention is directed to a capture assay to simultaneously screen for HBV, HCV and HIV nucleic acids in samples such as plasma. The nucleic acids including both viral DNA and RNA are purified from the plasma samples in a single extraction procedure. In one embodiment, a mixture of degenerate biotin-labelled PCR primers specific for the HBV, HCV, HIV-1 type M and HIV-1 type O are used to amplify any of these viruses which may be present in plasma. Amplified products are captured by hybridization to immobilized capture sequence, and thereafter detected. An internal control vector containing a synthetic fragment flanked by sequences corresponding to the HBV primers was designed to monitor sample recovery during extraction, amplification and detection. All major subtypes of HBV, HCV and HIV including HIV-1 type O have been confirmed and detected by the assay.

US Patent:

20050239108, Oct 27, 2005

Filed:

Feb 23, 2005

Appl. No.:

11/062809

Inventors:

Janet Barletta - Baltimore MD, US
Niel Constantine - Baltimore MD, US
Daniel Edelman - Baltimore MD, US
Mark Manak - Laurel MD, US
Jay Ji - Tucson AZ, US
Chang-Chih Tai - Potomac MD, US
William Highsmith - Rochester MN, US

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

The invention relates to methods and kits for detecting and/or monitoring biological molecules in a test sample. For example, the invention relates to methods and kits for detecting and/or monitoring HIV p24 antigen in human body fluid, biological toxins such as ricin or botulism in an environmental or biological sample, and prion protein from human, deer or bovine, such as PrP, in a biological sample. The antigen detection signal is boosted by amplification of a polynucleotide linked to a detector molecule using methods for nucleic acid amplification technology.

US Patent:

20070014690, Jan 18, 2007

Filed:

Jul 10, 2006

Appl. No.:

11/484293

Inventors:

Nathan Lawrence - Timonium MD, US
Feng Tao - Germantown MD, US
Allan Kakita - Huntington Beach CA, US
Mark Manak - Laurel MD, US
Richard Schumacher - South Easton MA, US
James Laugharn - Winchester MA, US

International Classification:

G01N 31/22

US Classification:

422058000

Abstract:

Devices and methods are described for homogenization, processing, detection, and analysis of biological samples such as insects, fungi, bacteria, and plant and animal tissues. Multiple chambers in these devices permit different processing functions to be carried out at each stage, such that the resulting homogenized product can be further processed, purified, analyzed, and/or biomolecules such as metabolites, proteins and nucleic acids, or pharmaceutical products can be detected. The device can be used in a hydrostatic pressure apparatus, in which different activities, i.e. incubations, addition or renewal of reagent, and generation and detection of signal can be carried out in the appropriate chamber. The method improves the preservation of biomolecules from chemical and enzymatic degradation relative to conventional means. Additionally, this method enables automated sample preparation and analytical processes.

US Patent:

56123418, Mar 18, 1997

Filed:

Jun 7, 1995

Appl. No.:

8/477939

Inventors:

Kuo-Hsiung Lee - Chapel Hill NC
Yoshiki Kashiwada - Niigata, JP
Lan Xie - Chapel Hill NC
Louis M. Cosentino - Springfield VA
Mark Manak - Laurel MD
Jing-Xi Xie - Beijing, CN
Yung-Chi Cheng - Woodbridge CT

Assignee:

Biotech Research Laboratories - Rockville MD
University of North Carolina at Chapel Hill - Chapel Hill NC

International Classification:

A61K 3136
C07D31762
C07D31764
C07D31766

US Classification:

514253

Abstract:

Disclosed herein are derivatives of 4,4'-dimethoxy-5,6,5'6'-bimethylenedioxy 2,2'-dimethoxycarbonyl biphenyl (DDB) which were synthesized and found to have anti-HIV activity. Of the compounds studied, 3,3'-dibromo DDB and 3-bromo-DDB initially exhibited the greatest anti-HIV activity.

US Patent:

58471650, Dec 8, 1998

Filed:

Feb 21, 1996

Appl. No.:

8/604305

Inventors:

Kuo-Hsiung Lee - Chapel Hill NC
Mark Cosentino - Springfield VA
Lan Xie - Chapel Hill NC
Mark Manak - Laurel MD

Assignee:

The University of North Carolina at Chapel Hill - Chapel Hill NC

International Classification:

C07D31178

US Classification:

549280

Abstract:

Disclosed are compounds of Formula I: ##STR1## wherein R. sup. 1 through R. sup. 6 are defined herein. Also disclosed are pharmaceutical compositions and methods of using these compositions for treating retroviral infections. Finally, a stereoselective method for synthesizing compounds of Formula I is described.

US Patent:

57262047, Mar 10, 1998

Filed:

Jun 5, 1995

Appl. No.:

8/462280

Inventors:

Kuo-Hsiung Lee - Chapel Hill NC
Yoshiki Kashiwada - Chapel Hill NC
Li Huang - Chapel Hill NC
Thomas Tung-Ying Lee - Chapel Hill NC
Mark Cosentino - Springfield VA
Jim Snider - Hagerstown MD
Mark Manak - Laurel MD
Lan Xie - Chapel Hill NC

Assignee:

Biotech Research Laboratories - Rockville MD
University of North Carolina at Chapel Hill - Chapel Hill NC

International Classification:

A61K 31365
C07D31194
C07D49306

US Classification:

514455

Abstract:

The present invention is directed to compounds that are analogs of the natural product suksdorfin. Compounds of the present invention include those having Formula I: ##STR1## wherein R. sup. 1, R. sup. 2, R. sup. 3, R. sup. 4, R. sup. 5 and R. sup. 6 are defined herein. The invention is also directed to methods of making these compounds, pharmaceutical compositions including these compounds and methods of using the compounds to inhibit retroviral infections in cells and tissues of animals. A method for stereoselectively preparing compounds within the scope of the invention by catalytic asymmetric dihydroxylation is also disclosed.

US Patent:

56798288, Oct 21, 1997

Filed:

Jun 5, 1995

Appl. No.:

8/463071

Inventors:

Kuo-Hsiung Lee - Chapel Hill NC
Yoshiki Kashiwada - Niigata, JP
Fumio Hashimoto - Kumamoto, JP
Louis Mark Cosentino - Springfield VA
Mark Manak - Laurel MD

Assignee:

Biotech Research Labs, Inc. - Rockville MD
University of North Carolina at Chapel Hill - Chapel Hill NC

International Classification:

C07C 69753
A61K 31505
A61K 3152
A61K 31215

US Classification:

560116

Abstract:

Some betulinic acid and dihydrobetulinic acid acyl derivatives according to the present invention have been found to have potent anti-HIV activity. Introducing a C. sub. 2 -C. sub. 20 substituted or unsubstituted acyl group at the C. sub. 3 -hydroxy group of betulinic acid and dihydrobetulinic acid produces the corresponding 3-O-acyl derivatives. The compounds of the present invention have the following formulae: ##STR1## where R may be a mono- or dicarboxylacyl group, substituted or unsubstituted, of from about 2 to about 20 carbon atoms, and R' may be hydrogen or a C. sub. 2 -C. sub. 10 substituted and unsubstituted alkyl or aryl group.