Feb 2011 to 2000 Chief Scientist, Department of Laboratory Diagnostics and MonitoringSERACARE LIFE SCIENCES, INC Gaithersburg, MD 2004 to 2010 Chief ScientistBBI BIOTECH Gaithersburg, MD 1992 to 2004 Senior Vice President and General ManagerCAMBRIDGE BIOTECH CORP Rockville, MD 1990 to 1992 Director Contracts & Services DivisionBIOTECH RESEARCH LABS. INC Rockville, MD 1981 to 1990 Director of Molecular BiologyJOHNS HOPKINS UNIVERSITY Baltimore, MD 1976 to 1981 Post Doctoral Fellow/Research Associate
Education:
University of Maryland College Park, MD 1992 MBA in Technical ManagementUniversity of Connecticut Storrs, CT 1976 Ph.D. in Biochemistry and BiophysicsUniversity of Connecticut Storrs, CT 1973 M.Sc. in Biochemistry and BiophysicsBoston University Boston, MA 1972 B.A. in Biology
Nathan Lawrence - Timonium MD, US Feng Tao - German Town MD, US Allan Kakita - Huntington Beach CA, US Mark Manak - Laurel MD, US Richard Schumacher - South Easton MA, US James Laugharn - Winchester MA, US
Devices and methods are described for homogenization, processing, detection, and analysis of biological samples such as insects, fungi, bacteria, and plant and animal tissues. Multiple chambers in these devices permit different processing functions to be carried out at each stage, such that the resulting homogenized product can be further processed, purified, analyzed, and/or biomolecules such as metabolites, proteins and nucleic acids, or pharmaceutical products can be detected. The device can be used in a hydrostatic pressure apparatus, in which different activities, i.e. incubations, addition or renewal of reagent, and generation and detection of signal can be carried out in the appropriate chamber. The method improves the preservation of biomolecules from chemical and enzymatic degradation relative to conventional means. Additionally, this method enables automated sample preparation and analytical processes.
Simultaneous Detection Of Hbv, Hcv, And Hiv In Plasma Samples Using A Multiplex Capture Assay
Jiuping Ji - Rockville MD, US Mark Manak - Laurel MD, US Irene Gonzalez - Baltimore MD, US
International Classification:
C12Q001/70 C12P019/34
US Classification:
435/005000, 435/091200
Abstract:
The present invention is directed to a capture assay to simultaneously screen for HBV, HCV and HIV nucleic acids in samples such as plasma. The nucleic acids including both viral DNA and RNA are purified from the plasma samples in a single extraction procedure. In one embodiment, a mixture of degenerate biotin-labelled PCR primers specific for the HBV, HCV, HIV-1 type M and HIV-1 type O are used to amplify any of these viruses which may be present in plasma. Amplified products are captured by hybridization to immobilized capture sequence, and thereafter detected. An internal control vector containing a synthetic fragment flanked by sequences corresponding to the HBV primers was designed to monitor sample recovery during extraction, amplification and detection. All major subtypes of HBV, HCV and HIV including HIV-1 type O have been confirmed and detected by the assay.
Immuno-Pcr Method For The Detection Of A Biomolecule In A Test Sample
Janet Barletta - Baltimore MD, US Niel Constantine - Baltimore MD, US Daniel Edelman - Baltimore MD, US Mark Manak - Laurel MD, US Jay Ji - Tucson AZ, US Chang-Chih Tai - Potomac MD, US William Highsmith - Rochester MN, US
International Classification:
C12Q001/68
US Classification:
435006000
Abstract:
The invention relates to methods and kits for detecting and/or monitoring biological molecules in a test sample. For example, the invention relates to methods and kits for detecting and/or monitoring HIV p24 antigen in human body fluid, biological toxins such as ricin or botulism in an environmental or biological sample, and prion protein from human, deer or bovine, such as PrP, in a biological sample. The antigen detection signal is boosted by amplification of a polynucleotide linked to a detector molecule using methods for nucleic acid amplification technology.
Multichamber Device For Processing Of Biological Samples Using High Pressure
Nathan Lawrence - Timonium MD, US Feng Tao - Germantown MD, US Allan Kakita - Huntington Beach CA, US Mark Manak - Laurel MD, US Richard Schumacher - South Easton MA, US James Laugharn - Winchester MA, US
International Classification:
G01N 31/22
US Classification:
422058000
Abstract:
Devices and methods are described for homogenization, processing, detection, and analysis of biological samples such as insects, fungi, bacteria, and plant and animal tissues. Multiple chambers in these devices permit different processing functions to be carried out at each stage, such that the resulting homogenized product can be further processed, purified, analyzed, and/or biomolecules such as metabolites, proteins and nucleic acids, or pharmaceutical products can be detected. The device can be used in a hydrostatic pressure apparatus, in which different activities, i.e. incubations, addition or renewal of reagent, and generation and detection of signal can be carried out in the appropriate chamber. The method improves the preservation of biomolecules from chemical and enzymatic degradation relative to conventional means. Additionally, this method enables automated sample preparation and analytical processes.
Kuo-Hsiung Lee - Chapel Hill NC Yoshiki Kashiwada - Niigata, JP Lan Xie - Chapel Hill NC Louis M. Cosentino - Springfield VA Mark Manak - Laurel MD Jing-Xi Xie - Beijing, CN Yung-Chi Cheng - Woodbridge CT
Assignee:
Biotech Research Laboratories - Rockville MD University of North Carolina at Chapel Hill - Chapel Hill NC
International Classification:
A61K 3136 C07D31762 C07D31764 C07D31766
US Classification:
514253
Abstract:
Disclosed herein are derivatives of 4,4'-dimethoxy-5,6,5'6'-bimethylenedioxy 2,2'-dimethoxycarbonyl biphenyl (DDB) which were synthesized and found to have anti-HIV activity. Of the compounds studied, 3,3'-dibromo DDB and 3-bromo-DDB initially exhibited the greatest anti-HIV activity.
Suksdorfin Analogs, Compositions Thereof, And Methods For Making And Using Thereof
Kuo-Hsiung Lee - Chapel Hill NC Mark Cosentino - Springfield VA Lan Xie - Chapel Hill NC Mark Manak - Laurel MD
Assignee:
The University of North Carolina at Chapel Hill - Chapel Hill NC
International Classification:
C07D31178
US Classification:
549280
Abstract:
Disclosed are compounds of Formula I: ##STR1## wherein R. sup. 1 through R. sup. 6 are defined herein. Also disclosed are pharmaceutical compositions and methods of using these compositions for treating retroviral infections. Finally, a stereoselective method for synthesizing compounds of Formula I is described.
Suksdorfin Analogs, Compositions Thereof, And Methods For Making And Using Thereof
Kuo-Hsiung Lee - Chapel Hill NC Yoshiki Kashiwada - Chapel Hill NC Li Huang - Chapel Hill NC Thomas Tung-Ying Lee - Chapel Hill NC Mark Cosentino - Springfield VA Jim Snider - Hagerstown MD Mark Manak - Laurel MD Lan Xie - Chapel Hill NC
Assignee:
Biotech Research Laboratories - Rockville MD University of North Carolina at Chapel Hill - Chapel Hill NC
International Classification:
A61K 31365 C07D31194 C07D49306
US Classification:
514455
Abstract:
The present invention is directed to compounds that are analogs of the natural product suksdorfin. Compounds of the present invention include those having Formula I: ##STR1## wherein R. sup. 1, R. sup. 2, R. sup. 3, R. sup. 4, R. sup. 5 and R. sup. 6 are defined herein. The invention is also directed to methods of making these compounds, pharmaceutical compositions including these compounds and methods of using the compounds to inhibit retroviral infections in cells and tissues of animals. A method for stereoselectively preparing compounds within the scope of the invention by catalytic asymmetric dihydroxylation is also disclosed.
Betulinic Acid And Dihydrobetulinic Acid Derivatives And Uses Therefor
Kuo-Hsiung Lee - Chapel Hill NC Yoshiki Kashiwada - Niigata, JP Fumio Hashimoto - Kumamoto, JP Louis Mark Cosentino - Springfield VA Mark Manak - Laurel MD
Assignee:
Biotech Research Labs, Inc. - Rockville MD University of North Carolina at Chapel Hill - Chapel Hill NC
International Classification:
C07C 69753 A61K 31505 A61K 3152 A61K 31215
US Classification:
560116
Abstract:
Some betulinic acid and dihydrobetulinic acid acyl derivatives according to the present invention have been found to have potent anti-HIV activity. Introducing a C. sub. 2 -C. sub. 20 substituted or unsubstituted acyl group at the C. sub. 3 -hydroxy group of betulinic acid and dihydrobetulinic acid produces the corresponding 3-O-acyl derivatives. The compounds of the present invention have the following formulae: ##STR1## where R may be a mono- or dicarboxylacyl group, substituted or unsubstituted, of from about 2 to about 20 carbon atoms, and R' may be hydrogen or a C. sub. 2 -C. sub. 10 substituted and unsubstituted alkyl or aryl group.